Document Detail


The influence of wavelength and probe configuration on findings of a skin vasoconstriction test when using laser Doppler perfusion devices.
MedLine Citation:
PMID:  16403539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to establish the degree to which a standardized test based on laser Doppler blood flow measurement is dependent on the particular equipment set-up being used. For this purpose, we examined finger skin blood flow with laser Doppler instruments in 20 healthy subjects. In laser Doppler perfusion monitoring (LDPM), we used a custom-made probe with two detecting fibers placed 0.25 and 1.2 mm from the illuminating fiber, respectively, and two laser Doppler perfusion imagers (LDPI) with a wavelength of 632.8 nm and 780 nm, respectively. Warming of the hand was achieved with a Peltier element, and reflex vasoconstriction was induced by immersing the other hand for 3 min into a water bath kept at 15 degrees C. As a measure for the change in skin blood flow, a vasoconstriction index (VAC: cooling/before cooling) was calculated and used for the comparison of the different devices. VAC values gathered around 0.6 for all devices. However, LDPI with a wavelength of 632.8 nm showed a slightly higher VAC index, and the difference was significant. We conclude that using a standardized test is the most appropriate for monitoring changes in blood flow rather than recording and comparing discrete values in intermittent recordings. Although a difference was noted when comparing the devices, different fiber separations and wavelengths seem then to be of little consequence.
Authors:
Carolin Freccero; Per Wollmer; Göran Sundkvist; Henry Svensson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-05
Journal Detail:
Title:  Microvascular research     Volume:  71     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-02-03     Completed Date:  2006-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  64-7     Citation Subset:  IM    
Affiliation:
Department of Plastic and Reconstructive Surgery, Malmö University Hospital, University of Lund, SE-20502 Malmö, Sweden. carolin.freccero@plastsurg.mas.lu.se
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MeSH Terms
Descriptor/Qualifier:
Adult
Fingers / blood supply
Humans
Laser-Doppler Flowmetry / instrumentation,  methods*
Male
Monitoring, Physiologic
Perfusion
Regional Blood Flow
Skin / blood supply*,  ultrasonography*
Skin Temperature
Skin Tests
Time Factors
Vasoconstriction / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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