Document Detail

The influence and utility of varying field strength for the separation of tryptic peptides by ion mobility-mass spectrometry.
MedLine Citation:
PMID:  15694766     Owner:  NLM     Status:  MEDLINE    
The influence of field strength on the separation of tryptic peptides by drift tube-based ion mobility-mass spectrometry is reported. Operating the ion mobility drift tube at elevated field strengths (expressed in V cm(-1) torr(-1)) reduces separation times and increases ion transmission efficiencies. Several accounts in the literature suggest that performing ion mobility separation at elevated field strength can change the selectivity of ion separation. To evaluate the field strength dependant selectivity of ion mobility separation, we examined a data set of 65 singly charged tryptic peptide ion signals (mass range 500-2500 m/z) at six different field strengths and four different drift gas compositions (He, N2, Ar, and CH4). Our results clearly illustrate that changing the field strength from low field (15 V cm(-1) torr(-1)) to high field (66 V cm(-1) torr(-1)) does not significantly alter the selectivity or peak capacity of IM-MS. The implications of these results are discussed in the context of separation methodologies that rely on the field strength dependence of ion mobility for separation selectivity, e.g., high-field asymmetric ion mobility spectrometry (FAIMS).
Brandon T Ruotolo; John A McLean; Kent J Gillig; David H Russell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  16     ISSN:  1044-0305     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-07     Completed Date:  2005-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158-65     Citation Subset:  IM    
Laboratory for Biological Mass Spectrometry, Department of Chemistry, Texas A&M University College Station, Texas 77843, USA.
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MeSH Terms
Amino Acid Sequence
Mass Spectrometry / methods*
Molecular Sequence Data
Peptide Fragments / analysis,  chemistry,  isolation & purification*,  metabolism*
Trypsin / metabolism*
Reg. No./Substance:
0/Peptide Fragments; EC

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