Document Detail

The influence of substrate and electron acceptor availability on bioactive zone dynamics in porous media.
MedLine Citation:
PMID:  14568400     Owner:  NLM     Status:  MEDLINE    
Two approaches were used to investigate the influence of dissolved oxygen (DO) and substrate availability on the formation and dynamics of "bioactive zones" in a water-saturated porous medium. A bioactive zone is defined as a region where a microbial community is sufficiently active to metabolize bioavailable substrates. In the first approach, microbial activity was characterized by monitoring the spatial and temporal variability of DO and aqueous substrate (salicylate and naphthalene) concentrations during miscible-displacement experiments. In the second approach, microbial activity was monitored using multiple fiber optics emplaced in the porous medium to detect luminescence produced by Pseudomonas putida RB1353, a bioluminescent reporter organism that produces light when salicylate (an intermediate of naphthalene degradation) is present. The results of both approaches show that the location and size of the bioactive zones were influenced by in situ DO and substrate availability. When DO was not a limiting factor (i.e., lower substrate input concentrations), the bioactive zone encompassed the entire column, with the majority of the microbial activity occurring between the inlet and midpoint. However, as the availability of DO became limiting for the higher substrate input experiments, the size of the bioactive zone shrank and was ultimately limited to the proximity of the column inlet.
Irfan Yolcubal; Jonathan G Dorn; Raina M Maier; Mark L Brusseau
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of contaminant hydrology     Volume:  66     ISSN:  0169-7722     ISO Abbreviation:  J. Contam. Hydrol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-21     Completed Date:  2004-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8805644     Medline TA:  J Contam Hydrol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  219-37     Citation Subset:  IM    
Department of Hydrology and Water Resources, University of Arizona, 429 Shantz, Tucson, AZ 85721, USA.
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MeSH Terms
Biodegradation, Environmental
Environmental Monitoring
Luminescent Measurements
Naphthalenes / metabolism
Oxygen / chemistry*
Pseudomonas putida / physiology
Soil Microbiology*
Soil Pollutants / metabolism*
Water Pollutants / metabolism*
Reg. No./Substance:
0/Naphthalenes; 0/Soil Pollutants; 0/Water Pollutants; 7782-44-7/Oxygen; 91-20-3/naphthalene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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