Document Detail

The influence of solvent composition on antioxidant potential of model polyphenols and red wines determined with DPPH.
MedLine Citation:
PMID:  23186019     Owner:  NLM     Status:  Publisher    
Solvent composition has a large influence on measured antioxidant potential (AOP) of model polyphenols and red wines with 2,2-diphenyl-1-picrylhydrazyl (DPPH). We have shown that incorporation of aqueous buffer in the assay medium results in higher reactivity of catechin and caftaric acid, that are among major polyphenolic constituents of wines. Consequently AOPs of red wines determined after 60 min incubation at 25 oC in buffered methanol are 1.5- to 1.6-fold of values determined in methanol. Even in buffered methanol wine polyphenols are not fully oxidized after 60 min. Only about half of Trolox equivalents were determined in comparison to Folin Ciocalteu assay. Buffer composition, pH, time of incubation, temperature and concentration of antioxidants and DPPH that all contribute to the resulting value of the AOP should be standardized or at least strictly reported as minor differences in experimental procedures can account for large variations in determined AOP with DPPH for same samples.
Laura Bertalanič; Tatjana Košmerl; Natasa Poklar Ulrih; Blaz Cigic
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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