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The influence of sex, menstrual cycle phase, estrogen, obesity, and endurance training on carbohydrate and lipid metabolism at rest and during moderate-intensity endurance exercise.
MedLine Citation:
PMID:  18641727     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Carbohydrate (CHO) and fat constitute the main fuels used during endurance exercise. The purpose of this thesis was to determine how sex, menstrual cycle phase, estrogen, obesity, and endurance training (ET) influence substrate metabolism. In study 1, men (n = 11) and women (n = 13) underwent primed constant infusion of [6,6-2H] glucose with muscle biopsies taken before and after a 90 min bike ride at 65% VO2 peak. Luteal-phase women had lower muscle glycogen utilization compared with follicular-phase women. Women had lower respiratory exchange ratio (RER) and plasma glucose utilization than men. In study 2, men (n = 17) and women (n = 19) had muscle biopsies taken before and after a 90 min bike ride at 65% VO2 peak. Women had greater intramyocellular lipid (IMCL) content and lower RER during exercise, with no effect of sex on net IMCL utilization. In study 3, Men (n = 11) were treated with 17-beta-estradiol (E2, 2 mg.d(-1), 8 d) and placebo and underwent primed constant infusion of [6,6-2H] glucose with muscle biopsies taken before and after a 90 min bike ride at 65% VO2 peak. E2 lowered RER and plasma glucose oxidation during exercise, with no effect on muscle glycogen utilization. In study 4, lean (n = 12) and obese (n = 11) women underwent 12 weeks of ET with muscle biopsies taken before and after training. Obese women were insulin resistant compared with lean women. ET increased oxidative capacity and beta-oxidation capacity, as well as mitochondrial content. ET redistributed IMCL from the subsarcolemmal region to the intermyofibrillar region of the muscle fibre. These studies demonstrate that substrate metabolism is affected by many factors in health and disease and the ability to manipulate these factors is of importance to athletes wishing to improve performance, as well as to obese individuals attempting to normalize an altered metabolic state.
Authors:
Michaela C Devries
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme     Volume:  33     ISSN:  1715-5312     ISO Abbreviation:  -     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264333     Medline TA:  Appl Physiol Nutr Metab     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  808-9     Citation Subset:  IM    
Affiliation:
Department of Pediatrics and Medicine, McMaster University, Hamilton, ON, Canada. devrimc@mcmaster.ca
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