| The influence of porcine pancreas digestion parameters and islet histomorphology on islet isolation outcome. | |
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MedLine Citation:
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PMID: 21721406 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Transplantation of the pig islets of Langerhans is considered as the future treatment for patients suffering from type I diabetes mellitus. Despite the adaptation of modified Ricordi method and highly purified collagenase, the results of pancreas digestions are precarious. Selection of proper donor and optimal digestion procedure are fundamental. The aim of this study was to assess the impact of pancreas procuring parameters on pig islets yield. The pancreata were harvested from 69 market sows weighting over 150 kg. After intraductal injection of cold collagenase solution pancreata were transported in UW solution or under conditions of two layer method (TLM). In laboratory pancreata were digested at 37 degrees C according to Ricordi isolation method or stationary in the bottle. The particular parameters of isolation procedure were considered as substantial. Pig weight, volume of infused collagenase solution, TLM application and pancreas dividing before digestion positively affected islet yield. Additionally, the influence of pancreatic islet tissue histomorphology on isolation outcome was studied. Proper donor selection as well as adequate digestion parameters could improve pig islet recovery during islet isolation. |
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Authors:
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J Kinasiewicz; M Sabat; M Antosiak-Iwańska; E Godlewska; E Sitarek; T Orłowski |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Polish journal of veterinary sciences Volume: 14 ISSN: 1505-1773 ISO Abbreviation: Pol J Vet Sci Publication Date: 2011 |
Date Detail:
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Created Date: 2011-07-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125473 Medline TA: Pol J Vet Sci Country: Poland |
Other Details:
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Languages: eng Pagination: 227-30 Citation Subset: IM |
Affiliation:
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Nałecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland. jkinasiewicz@ibib.waw.pl |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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