Document Detail

The influence of oxygen tension, temperature, and hemoglobin concentration on the rheologic properties of sickle erythrocytes.
MedLine Citation:
PMID:  2400812     Owner:  NLM     Status:  MEDLINE    
With the use of micromanipulation techniques, the shear modulus or "rigidity" mu, the recovery time tc, and the unfolding time tf for individual sickle cells have been measured at different oxygen tensions, temperatures, and cell densities. In these experiments, the partial pressure of oxygen was varied from 156 to 40 mm Hg and the temperature was controlled at 25 degrees C or 37 degrees C. Three mean cellular hemoglobin concentrations were studied: 29 g/dL, 33 g/dL, and 46 g/dL. The lighter cells (29 and 33 g/dL) exhibited at most a threefold increase in rigidity as the pO2 was decreased from 156 to 40 mm Hg. At 25 degrees C, the densest cells (46 g/dL) also exhibited a threefold increase. However, at 37 degrees C, the rigidity of these cells increased eightfold between 156 to 40 mm Hg. Compared with normal cells, this gives a rigidity that is 18 times larger. In contrast to the values for mu, the values for tc and tf remained essentially unchanged (within the accuracy of the experiments) for the lighter cells and could not be measured for the densest cells.
L H Mackie; R M Hochmuth
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  76     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-10-23     Completed Date:  1990-10-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1256-61     Citation Subset:  AIM; IM    
Department of Biomedical Engineering, Duke University, Durham, NC.
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MeSH Terms
Anemia, Sickle Cell / blood*
Erythrocytes, Abnormal / analysis,  physiology*
Hemoglobins / analysis,  physiology*
Middle Aged
Oxygen / physiology*
Grant Support
Reg. No./Substance:
0/Hemoglobins; 7782-44-7/Oxygen

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