Document Detail

The influence of oral lipid loads on acylation stimulating protein (ASP) in healthy volunteers.
MedLine Citation:
PMID:  9822948     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To examine the hypothesis that a sustained rise in plasma acylation stimulating protein (ASP, C3a desarg) accompanies the elevation in triacylglycerol that follows the ingestion of an oral fat load.
DESIGN: Following an overnight fast, blood samples were obtained from healthy volunteers while fasting and 15 min, 1, 2, 4, 6 and 8 h following ingestion of: (i) a liquid meal, rich in dairy fat (eight subjects) and (ii) a semi-liquid meal, with higher total fat content and rich in polyunsaturated fat (six subjects).
SUBJECTS AND METHODS: Four male and four female volunteers (age range: 22-51 y; body mass index (BMI): 17.9-26.9 kg/m2) received the first meal. Six subjects (age range: 32-60 y; BMI: 18.0-28.4 kg/m2), including three from the first study, received the second meal using the same protocol. ASP and C5a were measured by radioimmunoassay (RIA) and the complement proteins C3, factor B and C5 by radial immunodiffusion or nephelometry. Tumour necrosis factor (TNF)-alpha was measured by enhanced ELISA, and plasma cholesterol and triacylglycerol by an automated enzymatic method. The presence of chylomicrons was assessed in post-prandial plasma samples taken after the second meal.
RESULTS: There was no significant change in mean ASP concentration in either group at any time point, following ingestion of either meal. However, there was a significant positive linear trend in ASP following the second fat challenge (ANOVA; P < 0.05). There was also no change in complement proteins, plasma cholesterol or TNF-alpha. Plasma triacylglycerol rose significantly after the first and second meals (P < 0.05 and P < 0.001 at 2 h post-prandially); the mean maximum rise above the fasting level was 58 +/- 41% and 89 +/- 38% respectively (mean +/- s.d.). Chylomicrons were detected in samples taken from each subject after the second meal. Analysis of individual ASP data showed a sustained rise in one subject after the first meal and two subjects after the second meal. Substantial variation in ASP concentration was observed in samples taken in the first 2 h post-prandially.
CONCLUSION: There was no significant change in ASP nor other complement proteins for either group of subjects following ingestion of the lipid loads. Individual data showed substantial variation in post-prandial ASP, but multiple plasma sampling did not define the basis for this variation.
J A Charlesworth; P W Peake; L V Campbell; B A Pussell; S O'Grady; T Tzilopoulos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity     Volume:  22     ISSN:  -     ISO Abbreviation:  Int. J. Obes. Relat. Metab. Disord.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-12     Completed Date:  1999-01-12     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  9313169     Medline TA:  Int J Obes Relat Metab Disord     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1096-102     Citation Subset:  IM    
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MeSH Terms
Blood Proteins / metabolism*
Body Constitution
Body Mass Index
Complement C3a* / analogs & derivatives*
Dietary Fats / administration & dosage,  pharmacology*
Fatty Acids, Unsaturated / pharmacology
Lipids / blood
Middle Aged
Postprandial Period
Reference Values
Triglycerides / blood
Tumor Necrosis Factor-alpha / analysis
Reg. No./Substance:
0/Blood Proteins; 0/Dietary Fats; 0/Fatty Acids, Unsaturated; 0/Lipids; 0/Triglycerides; 0/Tumor Necrosis Factor-alpha; 0/complement C3a, des-Arg-(77)-; 80295-42-7/Complement C3a

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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