Document Detail


The influence of microcystin-LR on fish phagocytic cells.
MedLine Citation:
PMID:  17884965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro influence of microcystin-LR (MC-LR) on rainbow trout phagocytic cell functions was studied. The cells isolated from the fish blood were exposed on MC-LR at the concentrations of 1, 5, 10, 20 microg/mL. The viability of the cells after 2, 4 and 24 hour incubation with MC-LR was studied as well as phagocytic cell ability measured as zymozan particle phagocytosis and metabolic activity measured as respiratory burst activity. The toxin caused time- and dose-dependent viability decrease, the strongest after cell exposure on 10 and 20 microg/mL and observed only after 24 hours incubation when 1 microg/mL was applied. The phagocytic ability was elevated in the presence of MC-LR at the dose of 5 microg/mL. MC-LR had also modulatory influence on respiratory burst activity. While the two lower doses caused the stimulation of the parameter, the two higher ones diminished superoxide anion production in the triggered cells. The results show the potency of the toxin to influence the fish phagocytic cell functions.
Authors:
A Sierosławska; A Rymuszka; A Bownik; T Skowroński
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human & experimental toxicology     Volume:  26     ISSN:  0960-3271     ISO Abbreviation:  Hum Exp Toxicol     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-09-21     Completed Date:  2007-10-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004560     Medline TA:  Hum Exp Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  603-7     Citation Subset:  IM    
Affiliation:
The John Paul II Catholic University of Lublin, Department of Physiology and Ecotoxicology, Lublin, Poland. ansie@kul.lublin.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects
Dose-Response Relationship, Drug
Microcystins / toxicity*
Oncorhynchus mykiss / blood*
Phagocytes / drug effects*,  pathology
Phagocytosis / drug effects*
Respiratory Burst / drug effects*
Time Factors
Chemical
Reg. No./Substance:
0/Microcystins; 101043-37-2/cyanoginosin LR

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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