| The influence of microchannels on neurite growth and architecture. | |
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MedLine Citation:
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PMID: 15350782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microchannels were produced using a photolithographic technique to pattern polyimide walls (11 microm in height and 20-60 microm in width) onto a planar glass substrate. PC12 cells were seeded onto patterned surfaces. After 3 days of culture in NGF supplemented medium cells were viable and extended neurites. Culture in microchannels influenced the direction of neurite growth (theta Orientation) and the complexity of PC12 cell architecture including neurite length (L(Neurite)), the number of neurites emerging per cell (N(Neurites)), and the angle at which neurites emerged from the cell soma (theta Soma). In microchannels neurites oriented parallel to channel walls and the complexity of neuronal architecture was reduced. Both of these effects were strongest for cells located in channels 20-30 microm wide. Within each channel the magnitude of the effect on orientation and architecture was inversely proportional to the distance of the soma from the channel wall. Microtubule and actin filament mobility within the cytoplasm may underly effect on neurite orientation and cell architecture. By manipulating channel width the overall direction of neurite growth and the complexity of neuronal architecture was controlled. Results from these studies will be applied towards the development of biomaterials for microfluidic platforms and drug discovery studies and in neural regeneration research-two applications that would be significantly improved given the ability to control neurite orientation and the complexity of neuronal architecture. |
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Authors:
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Melissa J Mahoney; Ruth R Chen; Jian Tan; W Mark Saltzman |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biomaterials Volume: 26 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2004-09-07 Completed Date: 2005-03-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 771-8 Citation Subset: IM |
Affiliation:
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School of Chemical Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, USA. mahonem@colorado.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / chemistry* Cell Line Cell Polarity Cell Proliferation Materials Testing Microfluidic Analytical Techniques / instrumentation, methods* Neurites / physiology*, ultrastructure* Neurons / cytology*, physiology* Rats Resins, Synthetic Surface Properties Tissue Engineering / methods* |
| Grant Support | |
ID/Acronym/Agency:
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DE-14097/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Resins, Synthetic; 39355-34-5/polyimide resin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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