Document Detail

The influence of linoleic and linolenic acid on the activity and intracellular localisation of phospholipase D in COS-1 cells.
MedLine Citation:
PMID:  19090716     Owner:  NLM     Status:  MEDLINE    
Phospholipase D (PLD) is a receptor-regulated signalling enzyme involved in biological functions, such as exocytosis, phagocytosis, actin dynamics, membrane trafficking, and is considered to be essential for stimulated degranulation of cells. The purpose of our investigation was to examine how the fatty acid pattern of cellular membranes influences the activities and cellular distribution of the PLD1 and PLD2 isoforms. Expression of GFP-tagged PLD1 and PLD2 in COS-1 cells that were stimulated with mastoparan after cultivation in 20 micromol linoleic (C18:2n6) or linolenic (C18:3n3) acid for 4 d demonstrated that PLD1 dramatically alters its cellular distribution and is redistributed from intracellular vesicles to the cell surface. PLD2, on the other hand, maintains its localisation at the plasma membrane. The activity of PLD, which corresponds to PLD1 and PLD2, significantly increased two- to three-fold in the presence of the fatty acids. We conclude that linoleic acid and linolenic acid supplementation affect the intracellular trafficking of the PLD1 isoform and the activity of PLD most likely due to alterations in the membrane lipid environment conferred by the fatty acids.
Anja Gemeinhardt; Marwan Alfalah; Thomas Gück; Hassan Y Naim; Herbert Fuhrmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry     Volume:  390     ISSN:  1431-6730     ISO Abbreviation:  Biol. Chem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  253-8     Citation Subset:  IM    
Institute of Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany.
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MeSH Terms
COS Cells
Cercopithecus aethiops
Linoleic Acid / pharmacology*
Phospholipase D / metabolism*
alpha-Linolenic Acid / pharmacology*
Reg. No./Substance:
2197-37-7/Linoleic Acid; 463-40-1/alpha-Linolenic Acid; EC D

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