Document Detail

The influence of ketoconazole on the activity of asparagine protease produced by Candida strains.
MedLine Citation:
PMID:  17564323     Owner:  NLM     Status:  MEDLINE    
The investigations concerning the activity of antimycotic drugs on pathogenic fungi, revealed a decrease of sensitivity of fungi, especially of the Candida species. In other investigations we have stated that strains isolated from patients have other than standard enzymatic activity. So it seems to be interesting to have evaluated the model for analysis in vitro the influence of antimycotic drug for egzoenzyme of fungi. The aims of the study were: 1. estimation of the influence in vitro ketoconazole, which was added everyday for 3 weeks on curves of activity of the drug, on strains isolated from patients; 2. estimation of asparagine protease activity on strains before, during and after the added drug to the culture. During the study we evaluated the model in vitro to obtain strains with lower sensitivity for a antimycotic drug. Everyday during a 3 week period we added to the culture ketoconazole in the concentration of about 2, 4 and 6 times higher than the minimal inhibitory concentration (MIC). Before, 1, 2 and 3 weeks after we investigated the activity of asparaginee protease. We obtained the following results: 1. Ketoconazole added to the culture of Candida strains during 3 weeks had influenced the response curves, which was observed as statistical significant changes between MIC values. 2. Ketoconazole after 1 week of administration decreased and after 2 and 3 weeks increased of asparagine protease activity.
Piotr Kurnatowski; Arkadiusz Grabek
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research communications in molecular pathology and pharmacology     Volume:  115-116     ISSN:  1078-0297     ISO Abbreviation:  Res. Commun. Mol. Pathol. Pharmacol.     Publication Date:  2004  
Date Detail:
Created Date:  2007-06-13     Completed Date:  2007-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9437512     Medline TA:  Res Commun Mol Pathol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  265-76     Citation Subset:  IM    
Department of Biology and Medical Parasitology, Medical University of L6di, Hallera 1, Lódź, Poland.
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MeSH Terms
Antifungal Agents / pharmacology*
Asparagine / metabolism*
Candida / drug effects*,  enzymology*,  genetics
Endopeptidases / metabolism*
Ketoconazole / pharmacology*
Microbial Sensitivity Tests
Species Specificity
Reg. No./Substance:
0/Antifungal Agents; 65277-42-1/Ketoconazole; 7006-34-0/Asparagine; EC 3.4.-/Endopeptidases

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