| The influence of increasing media methanol concentration on sophorolipid biosynthesis from glycerol-based feedstocks. | |
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MedLine Citation:
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PMID: 20490613 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Candida bombicola, a known producer of sophorolipids (SLs; glycolipid surfactants), was grown on glycerol and oleic acid with up to 1.5% (v/v) methanol in the fermentation growth media to assess the effects of methanol presence on SL synthesis and structural distribution. Increasing methanol concentrations had little effect on the growth of the organism resulting in average cell dry weights (CDW; after SL separation) of 20.8 ± 0.7 g/l between 0 and 1.5% methanol. However, increasing methanol concentrations decreased SL production by 56% (from 12.7 to 5.6 g/l at 1.5% methanol) which translated to SL yields on a cellular basis of between 0.60 g SL/g cells (in the absence of methanol) to 0.27 g SL/g cells (in the presence of 1.5% methanol). LC/MS revealed that increased methanol concentrations also resulted in larger concentrations (up to 20 mol%) of free acid SLs but had little effect on the ratios of diacetylated SL lactones synthesized with palmitic acid (4 mol%), linoleic acid (3 mol%), oleic acid (80 mol%), and stearic acid (13 mol%) as the hydrophobic moieties. |
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Authors:
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Richard D Ashby; Daniel K Y Solaiman |
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Publication Detail:
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Type: Journal Article Date: 2010-05-21 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-17 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1429-37 Citation Subset: IM |
Affiliation:
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US Department of Agriculture, Fats, Oils and Animal Coproducts Research Unit, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. Rick.Ashby@ars.usda.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biomass Candida / drug effects*, growth & development, metabolism* Culture Media / chemistry Fermentation Glycerol / metabolism* Lipid Metabolism* Methanol / metabolism* Surface-Active Agents / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Surface-Active Agents; 56-81-5/Glycerol; 67-56-1/Methanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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