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The influence of hydrostatic pressure and urethane on the thermal inactivation of bacteriophage.
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MedLine Citation:
PMID:  18139005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Difco nutrient broth, containing 0.5 per cent NaCl, pH 6.6, Escherichia coli phages T1, T2, and T5 were inactivated at 66 degrees C., and T7 at 60 degrees C., at nearly the same rate. In each case the rate of destruction was not uniform but more or less decreased with time of heating. With T2 there was an initial increase in number of infective centers after heating for several minutes at 66 degrees C. Hydrostatic pressures up to 10,000 pounds per square inch retarded the thermal destruction of T1, T2, and T5, but accelerated that of T7, while small concentrations of urethane accelerated the rate of each. The rate of inactivation was increased by the addition of 0.005 M phosphate, and was decreased by 0.005 M MgCl(2) in all but T7, whose rate was unaffected by this amount of Mg. The influence of Ca was similar to that of Mg. The addition of 0.005 M MgCl(2) to the broth medium resulted in a first order rate of destruction of T5 at either normal or increased pressure, and with as well as without urethane. Analysis of data obtained under these conditions indicated that the thermal inactivation proceeds with a volume increase of activation of 113 cc. per mol, and with a heat and entropy of 170,000 calories and 425 E. U., respectively, in the rate-limiting reaction. In the presence of 0.1 M urethane the heat and volume change of activation are apparently slightly greater. The relation between concentration of urethane and the amount of acceleration in rate of destruction at normal pressure and 66 degrees C. indicated that the total rate involves at least two first order rate processes: the thermal inactivation itself and a urethane-catalyzed reaction, the latter involving the combination of an average of 2.3 molecules of urethane in the activated state of the bacteriophage molecule whose destruction results in loss of phage activity.
Authors:
R A C FOSTER; F H JOHNSON; V K MILLER
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  33     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1949 Sep 
Date Detail:
Created Date:  1949-12-01     Completed Date:  2007-12-27     Revised Date:  2013-02-19    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages*
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1949, by The Rockefeller Institute for Medical Research
Received Day: 16 Month: 3 Year: 1949
Print publication date: Day: 20 Month: 9 Year: 1949
Volume: 33 Issue: 1
First Page: 1 Last Page: 16
ID: 2147142
PubMed Id: 18139005

THE INFLUENCE OF HYDROSTATIC PRESSURE AND URETHANE ON THE THERMAL INACTIVATION OF BACTERIOPHAGE
Ruth A. C. Foster
Frank H. Johnson
Virginia K. Miller
From the Biological Laboratory, Princeton University, Princeton, New Jersey


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