Document Detail


The influence of film composition on the morphology, mechanical properties and surfactant recovery of phase-separated phospholipid-perfluorinated fatty acid mixed monolayers.
MedLine Citation:
PMID:  23043367     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Monolayer surfactant films comprised of a mixture of phospholipids and perfluorinated (or partially fluorinated) surfactants are of potential utility for applications in pulmonary lung surfactant-based therapies. As a simple, minimal model of such a lung surfactant system, binary mixed monolayer films comprised of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and perfluorooctadecanoic acid (C18F) prepared on a simplified lung fluid mimic subphase (pH 7.4, 150 mM NaCl) have been characterized in terms of mixing thermodynamics and compressibility (measured through π-A compression isotherms), film morphology (via atomic force, fluorescence and Brewster angle microscopy), as well as spreading rate and hysteresis response to repeated expansion-contraction cycles for a variety of compositions of mixed films. Under all mixing conditions, films and their components were found to be completely immiscible and phase-separated, though there were significant changes in the aforementioned film properties as a function of composition. Of particular note was the existence of a maximum in the extent of immiscibility (characterized by ∆G_ex^π values) and enhanced surfactant recovery during hysteresis experiments at ΧC18F > 0.30. The latter was attributed to the relatively rapid re-spreading rate of the perfluorinated amphiphile in comparison with DPPC alone at the air-water interface, which enhances the performance of this mixture as a potential pulmonary lung surfactant. Further, monolayer film structure could be tracked dynamically as a function of compression at the air-water interface via Brewster angle microscopy, with the C18F component being preferentially squeezed out of the film with compression, but returning rapidly upon re-expansion. In general, addition of C18F to DPPC monolayers resulted in improvements to mechanical, structural and re-spreading properties of the film, indicating the potential value of these compounds as additives to pulmonary lung surfactant formulations.
Authors:
Ala Eftaiha; Sophie Brunet; Matthew F Paige
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-8
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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