Document Detail


The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth.
MedLine Citation:
PMID:  18819656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.
Authors:
U Salma; M S M Rahman; S Islam; N Haque; T A Jubair; A K M F Haque; I J Mukti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pakistan journal of biological sciences: PJBS     Volume:  11     ISSN:  1028-8880     ISO Abbreviation:  Pak. J. Biol. Sci.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-09-29     Completed Date:  2008-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101247723     Medline TA:  Pak J Biol Sci     Country:  Pakistan    
Other Details:
Languages:  eng     Pagination:  1638-41     Citation Subset:  IM    
Affiliation:
Department of RDDR, Modern Herbal Group, Dhaka-1217, Bangladesh.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / chemistry
Cell Communication / drug effects
Cell Differentiation / drug effects
Genotype
Hydrogen-Ion Concentration
Plant Growth Regulators / metabolism*,  pharmacology
Plant Roots / metabolism
Plant Shoots / metabolism
Rauwolfia / drug effects*,  physiology
Regeneration / drug effects
Temperature
Time Factors
Tissue Culture Techniques
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Plant Growth Regulators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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