| The influence of diets and gut microflora on lectin binding patterns of intestinal mucins in rats. | |
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MedLine Citation:
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PMID: 7474928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The mechanisms responsible for the biosynthesis, storage, secretion, or degradation of intestinal mucins are still unclear. Little is known about the carbohydrate composition of mucins in response to changes in the intestinal lumen, so lectin histochemical techniques were used to study the alterations in glycoconjugate synthesis of mucins in rats under different diets and microfloras. EXPERIMENTAL DESIGN: Nine-week-old germ-free and conventional rats were given either a purified diet of finely powdered ingredients, including cellulose as a source of fiber, or a more coarsely ground commercial diet of natural ingredients containing crude fiber of cereal origin. To mimic the human situation more closely, a group of rats born germ-free, inoculated with a suspension of human feces, and fed a purified diet were used as an experimental model. RESULTS: In rats fed a commercial diet, the surface goblet cells in the small intestine were more intensely labeled with N-acetyl-glucosamine and sialic acid-linked D-galactose-specific lectins than in rats fed the purified diet. A similar increased staining with a N-acetylgalactosamine-specific lectin was observed in the large intestine of rats fed a commercial diet. The microbial flora modified the crypt-surface glycosylation of fucosyl and sialic acid residues in the large intestine. The human flora specifically altered the goblet cell glycoconjugates in the surface epithelium. CONCLUSIONS: The significant changes in goblet cell glycoconjugates reflect the adaptation of the intestinal mucosa to different diets and microbial populations. An overall reduction in sialic acid-linked D-galactose residues in conventional rats and a loss of crypt-to-surface gradient of fucosyl expression in the large intestine of human flora rats are likely to be due to differing strains of glycosidases in the two microflora. |
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Authors:
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R Sharma; U Schumacher |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 73 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1995-11-27 Completed Date: 1995-11-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 558-64 Citation Subset: IM |
Affiliation:
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Department of Human Morphology, University of Southampton, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosamine
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metabolism Animals Carbohydrate Sequence Cellulose / pharmacology Colon / chemistry, cytology, microbiology* Dietary Fiber / pharmacology* Germ-Free Life Glycoconjugates / analysis, metabolism Humans Immunohistochemistry Intestinal Mucosa / chemistry, cytology, microbiology Jejunum / chemistry, cytology, microbiology* Lectins / chemistry, metabolism* Male Molecular Sequence Data Mucins / analysis, metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Glycoconjugates; 0/Lectins; 0/Mucins; 7512-17-6/Acetylglucosamine; 9004-34-6/Cellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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