| The influence of curcumin on the cell cycle of HL-60 cells and contrast study. | |
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MedLine Citation:
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PMID: 12845723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanisms of curcumin inhibiting the proliferation of HL-60 cells were investigated. Acute myeloid leukemic cell line HL-60 was studied by using cell culture, NBT reduction, SABC method measuring BrdU incorporation rate, FCM measuring DNA contents and TUNEL method determining apoptotic cell percentage. Curcumin inhibited proliferation of HL-60 cells in a dose- and time-dependent manner. When the HL-60 cells were treated with 25 mumol/L curcumin for 48 h, the inhibitory rate was 60.71% +/- 1.20%. The study on BrdU incorporation rate and the distribution of DNA content and NBT reduction indicated that curcumin arrested the cells in G2/M phase of cell cycle at first, then in G0/G1 phase, the whole cell cycle progression was slowed down and DNA synthesis activities was halted. The arrested cells went to apoptosis instead of differentiation. The comparative study indicated that the ability of curcumin regulating the cell cycle of HL-60 cells was stronger than As2O3 and the acting points of curcumin were not completely consistent with those of VP-16. It was suggested that curcumin was able to regulate, to some extent, the G1/S and G2/M transmit checkpoints and disturb the HL-60 cell cycle to induce apoptosis. |
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Authors:
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Y Wu; Y Chen; M He |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Tongji Medical University = Tong ji yi ke da xue xue bao Volume: 20 ISSN: 0257-716X ISO Abbreviation: J. Tongji Med. Univ. Publication Date: 2000 |
Date Detail:
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Created Date: 2003-07-08 Completed Date: 2003-12-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8605495 Medline TA: J Tongji Med Univ Country: China |
Other Details:
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Languages: eng Pagination: 123-5 Citation Subset: IM |
Affiliation:
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Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan 430022. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis / drug effects* Cell Cycle / drug effects Cell Division / drug effects Curcumin / pharmacology* DNA, Neoplasm / biosynthesis HL-60 Cells / pathology Humans |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/DNA, Neoplasm; 458-37-7/Curcumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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