| The influence of collagen film nanostructure on pulmonary stem cells and collagen-stromal cell interactions. | |
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MedLine Citation:
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PMID: 20673998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have recently identified a rare subpopulation of lung colony cells with the characteristics of pulmonary stem cells, and discovered that stem cell colonies grew preferentially on type I collagen films in a serum-free medium. In order to further optimize culture conditions and determine stem cell growth in relation to microenvironments (including the stroma, medium and nanostructures of type I collagen films), both primary and pre-sorted stem cells were cultured on the type I collagen films with controllable degree of polymerization and film thickness, as confirmed by an atomic force microscope and surface profiler. We found that in a primary culture, the spreading of stromal cells is greatly restrained and both the size and number of colonies are significantly reduced on highly polymerized collagen films. In contrast, in a pre-sorted stem cell culture without stromal cells, the intrinsic stem cell properties and cell number are independent of the degree of collagen polymerization. Our results indicate that the nanostructures of type I collagen films primarily affect stem colony formation through the collagen-stroma interactions. In those cases, collagen film thickness shows no effect on colony formation. |
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Authors:
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Chun-Jen Huang; Yi-Lun Chien; Tai-Yen Ling; Huan-Chien Cho; John Yu; Ying-Chih Chang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-01 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 8271-80 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Genomics Research Center, Academia Sinica, Taipei 115, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Biocompatible Materials / chemistry*, metabolism Cell Count Cell Size Cells, Cultured Collagen Type I / chemistry*, metabolism Lung / cytology* Mice Microscopy, Atomic Force Nanostructures / chemistry*, ultrastructure Octamer Transcription Factor-3 / metabolism Polymerization Serum / metabolism Stem Cells / cytology*, metabolism Stromal Cells / cytology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Biocompatible Materials; 0/Collagen Type I; 0/Octamer Transcription Factor-3; 0/alpha-smooth muscle actin, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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