Document Detail

The influence of choline chloride on murine hematopoiesis in vivo and in vitro.
MedLine Citation:
PMID:  6672108     Owner:  NLM     Status:  MEDLINE    
We report here studies to further document the ability of cholinergic agonists to influence murine hematopoiesis. B6C3F1 mice were administered ultrapure choline (0.14, 1.4, and 7.0 mg/animal) i.p. for three days, then randomly sacrificed for assessment of hematological parameters. No significant change was observed in the packed red cell volume; however, the WBC was elevated significantly (85-400%) over noncholine administered controls during all days examined [1-12]. In vitro choline (10 mM) increased heterogeneous marrow-derived CFU-GM colony formation 210% above controls. Nonadherent marrow cell-derived CFU-GM produced a significant elevation in colony formation (36%), suggesting choline may influence directly a portion of the CFU-GM population. A twofold increase in the CFU-GM kill after 16 mM hydroxyurea exposure suggests choline can stimulate a subpopulation of CFU-GM not normally in cell cycle. In addition, choline (10 mM) was effective in stimulating CSF activity from marrow-derived conditioned medium. Dose-response studies demonstrated that choline was also stimulatory for megakaryocyte colony formation (CFU-Mk), 26% at 5 mM. Erythroid precursor stem cells, CFU-E/BFU-E, were reduced significantly (10-50%) at all choline concentrations tested (1-50 mM). These studies document the ability of choline to influence hematopoiesis, and provide further evidence that cholinergic mechanisms may be important in the control of steady-state hematopoiesis.
V S Gallicchio; M G Chen; C Gamba-Vitalo; T D Watts
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cell cloning     Volume:  1     ISSN:  0737-1454     ISO Abbreviation:  Int. J. Cell Cloning     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-05-23     Completed Date:  1984-05-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8308172     Medline TA:  Int J Cell Cloning     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  451-63     Citation Subset:  IM    
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MeSH Terms
Choline / pharmacology*
Colony-Forming Units Assay
Hematopoiesis / drug effects*
Hematopoietic Stem Cells / drug effects*
Hydroxyurea / pharmacology
Grant Support
Reg. No./Substance:
127-07-1/Hydroxyurea; 62-49-7/Choline

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