Document Detail

The influence of catecholamines on pseudocholinesterase enzymatic activity. Results of a laboratory investigation.
MedLine Citation:
PMID:  16953463     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Acceleratory and inhibitory receptors have been described on the pseudocholinesterase (PCHE) molecule. An increased PCHE activity has been reported in patients with chronic pain and anxiety, conditions known to be correlated with increased plasma catecholamine levels. Aim of this laboratory investigation was to determine whether catecholamines have an effect on PCHE activity, as this knowledge could help to define the role of PCHE in the assessment of stress. METHODS: After Ethics committee approval and written informed consent, 3 ml of blood was collected from five healthy volunteers. Fourteen samples of 50 mul each were prepared from each of the volunteer's plasma. Epinephrine (25, 50, 100, 200, 400 and 1000 pg) and norepinephrine (50, 100, 200, 400, 800 and 2000 pg) were added to samples of each subject. Sodium-chloride solution was added to control samples. PCHE activity was photometrically assessed. RESULTS: PCHE activity was significantly higher after the addition of epinephrine (median 8304 versus 7386 U/l). This effect was not dose-dependent. PCHE activity did not change after addition of norepinephine. CONCLUSIONS: This mechanism might explain previous findings that showed higher levels of PCHE activity in the presence of chronic pain and anxiety. In the absence of a dose-response curve in the concentration range studied, PCHE activity does not appear to be suitable for the assessment of levels of stress.
Thomas Ledowski; Michael J Paech; Michael Clarke; Stephan A Schug
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2006-09-05
Journal Detail:
Title:  Journal of clinical monitoring and computing     Volume:  20     ISSN:  1387-1307     ISO Abbreviation:  J Clin Monit Comput     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-31     Completed Date:  2007-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806357     Medline TA:  J Clin Monit Comput     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  329-32     Citation Subset:  IM    
Department of Anaesthesia and Pain Medicine, Royal Perth Hospital, Wellington Street Campus, Perth, WA 6000, Australia.
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MeSH Terms
Anxiety / enzymology
Dose-Response Relationship, Drug
Epinephrine / administration & dosage,  pharmacology*
Norepinephrine / administration & dosage,  pharmacology*
Pain / enzymology
Pseudocholinesterase / blood*
Reg. No./Substance:
51-41-2/Norepinephrine; 51-43-4/Epinephrine; EC 3.1.1.-/Pseudocholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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