Document Detail


The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats.
MedLine Citation:
PMID:  8562319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although treatment with agents that block leukocyte function, including anti-ICAM-1 and doxycycline, reduces experimental central nervous system (CNS) ischemic injury, it is not known how leukocyte subset accumulation is affected by these agents. Using the rat two-vessel occlusion model and immunohistochemistry, we investigated granulocyte (PMN) and monocyte/macrophage (M phi) accumulation at 1 and 4 d postischemia. A total of 24 animals were randomized to sham surgery, or to ischemia with saline, anti-ICAM-1, or doxycycline treatments. No leukocytes were observed in sham animals. At 24 h postischemia, there was a moderate infiltration of PMN and M phi in untreated animals that was significantly decreased with either treatment. At 4 d after ischemia no PMN were identified, with extensive M phi accumulation occurring in untreated animals that was only partially reduced with doxycycline treatment. These results confirm that both anti-ICAM-1 and doxycycline treatments reduce PMN and M phi infiltration at 24 h. Delayed M phi accumulation occurs despite treatment, suggesting that some of these cells represent transformed resident microglia.
Authors:
W M Clark; J D Lauten; N Lessov; W Woodward; B M Coull
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  6     ISSN:  0895-8696     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  1995  
Date Detail:
Created Date:  1996-03-07     Completed Date:  1996-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  43-50     Citation Subset:  IM    
Affiliation:
Oregon Stroke Center, Department of Neurology, Oregon Health Sciences University, Portland, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology
Cell Adhesion / immunology
Cerebral Cortex / blood supply,  cytology,  immunology*
Doxycycline / pharmacology
Immunohistochemistry
Leukocyte Count
Leukocytes / cytology*,  immunology
Macrophages / cytology,  immunology
Male
Monocytes / cytology,  immunology
Neuroprotective Agents / pharmacology
Neutrophils / cytology,  immunology
Rats
Rats, Sprague-Dawley
Reperfusion Injury / immunology*
Grant Support
ID/Acronym/Agency:
2PO1 NS17493-08/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Neuroprotective Agents; 564-25-0/Doxycycline

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