| The influence of ageing in the cerebrospinal fluid concentrations of proteins that are derived from the choroid plexus, brain, and plasma. | |
| | |
MedLine Citation:
|
PMID: 22532968 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Studies have shown that ageing alone can cause increases in the concentrations of many cerebrospinal fluid (CSF) proteins. Therefore, CSF protein concentrations must be interpreted with caution before concluding that the increased concentrations of certain proteins can be used as disease-specific biomarkers. Age-related reduction in CSF turnover has been shown to have a significant concentrating effect on CSF proteins from young to old. As a result, CSF protein concentrations need to be corrected with age-specific turnovers first before performing any data comparisons between different ages. This study applied the concept of CSF/plasma concentration ratios of plasma-derived proteins that is frequently used in the investigation of brain barrier integrity to calculate the amount of protein that enters the CSF from the plasma side in different age groups. Based on our calculations, proteins with molecular weights greater than 91.92 kDa for the young, 109.51 kDa for the middle-aged and 120 kDa for the old should not be able to cross the brain barriers of the blood-brain and blood-CSF barriers to enter the CSF from the plasma side. For proteins that can be derived from the choroid plexus (CP), brain, and plasma, the amount that crosses the barriers to enter the CSF from the plasma side will contribute to their measured total protein concentrations in the CSF. CP and brain production of these proteins can be calculated when turnover corrected CSF protein concentrations are further corrected by the amount of protein that crosses the barriers. In this study, CP and brain produced concentrations of transthyretin, retinol binding protein, alpha-1-antitrypsin, gelsolin, and lactotransferrin were calculated. The production of these proteins decreased with age with alpha-1-antitrypsin protein revealing the most substantial decrease of 86% from young (0.14±0.01 mg·dL(-1)) to old (0.02 mg·dL(-1)). In conclusion, measured CSF protein concentrations for proteins that can be derived from the CP, brain, and plasma need to be corrected by age-specific CSF turnovers and by the amount of protein that crosses the brain barriers first before their concentrations can be compared logically between different ages. |
| | |
Authors:
|
Carl P C Chen; Ruo Li Chen; Jane E Preston |
Related Documents
:
|
18045498 - Phylogenomics and signature proteins for the alpha proteobacteria and its main groups. 7620988 - A comprehensive representation of extensive similarity linkage between large numbers of... 18586748 - Machos: markov clusters of homologous subsequences. 17060158 - Identification of essential loops and residues of glucosyltransferase v (gtrv) of shige... 10595578 - The essential role of mitochondria in the biogenesis of cellular iron-sulfur proteins. 10355358 - Enigmatic relationship of pleckstrin homology domain with phospholipid breakdown mediat... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Experimental gerontology Volume: 47 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-04-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
|
Languages: eng Pagination: 323-8 Citation Subset: IM |
Affiliation:
|
Department of Physical Medicine & Rehabilitation, Chang Gung Memorial Hospital at Linkou and Chang Gung University College of Medicine, Taiwan. carlchendr@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
BB/D01381X/1//Biotechnology and Biological Sciences Research Council |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vasorelaxation to capsaicin and its effects on calcium influx in arteries.
Next Document: The role of vitamin D in asthma.