Document Detail

The inferior colliculus of GEPRs contains greater numbers of cells that express glutamate decarboxylase (GAD67) mRNA.
MedLine Citation:
PMID:  8453948     Owner:  NLM     Status:  MEDLINE    
Previous studies have shown significantly greater GABA levels and numbers of GABAergic neurons in the central nucleus of the inferior colliculus (ICCN) of genetically epilepsy-prone rats (GEPR-9s). In the present study, in situ hybridization and emulsion autoradiographic techniques were used to determine whether there are also elevated numbers of ICCN cells that contain the 67-kD form of mRNA for the GABA synthesizing enzyme, glutamate decarboxylase (GAD), in GEPR-9s as compared to normal Sprague-Dawley (SD) rats. Hybridization with a 35S-labeled RNA probe complementary to a span of monkey GAD mRNA labeled cells throughout the brain including the ICCN. Labeled cells in the ICCN appeared to be of different sizes that corresponded with previous descriptions of GABAergic neurons from immunocytochemical studies. In the GEPR-9s, a larger number of GAD67 cRNA labeled neurons was observed in the ICCN as compared to SD rats. The external nucleus of the inferior colliculus was also found to contain significantly greater numbers of GAD67 cRNA labeled neurons whereas in the frontal cortex, a region of the brain that is not required for audiogenic seizure activity in GEPR-9s, there were no significant differences in hybridization between GEPR-9s and SD rats. Interestingly, within the superficial layers of the superior colliculus there was a higher density of hybridization in GEPR-9s than in SD rats indicating higher levels of GAD expression.(ABSTRACT TRUNCATED AT 250 WORDS)
C E Ribak; J C Lauterborn; M S Navetta; C M Gall
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Epilepsy research     Volume:  14     ISSN:  0920-1211     ISO Abbreviation:  Epilepsy Res.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-21     Completed Date:  1993-04-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8703089     Medline TA:  Epilepsy Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
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MeSH Terms
Epilepsy / enzymology*,  genetics
Glutamate Decarboxylase / metabolism*
In Situ Hybridization
Inferior Colliculi / enzymology*
Neurons / enzymology
RNA Probes
RNA, Messenger / analysis*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/RNA Probes; 0/RNA, Messenger; EC Decarboxylase

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