| The inferior colliculus of GEPRs contains greater numbers of cells that express glutamate decarboxylase (GAD67) mRNA. | |
| | |
MedLine Citation:
|
PMID: 8453948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previous studies have shown significantly greater GABA levels and numbers of GABAergic neurons in the central nucleus of the inferior colliculus (ICCN) of genetically epilepsy-prone rats (GEPR-9s). In the present study, in situ hybridization and emulsion autoradiographic techniques were used to determine whether there are also elevated numbers of ICCN cells that contain the 67-kD form of mRNA for the GABA synthesizing enzyme, glutamate decarboxylase (GAD), in GEPR-9s as compared to normal Sprague-Dawley (SD) rats. Hybridization with a 35S-labeled RNA probe complementary to a span of monkey GAD mRNA labeled cells throughout the brain including the ICCN. Labeled cells in the ICCN appeared to be of different sizes that corresponded with previous descriptions of GABAergic neurons from immunocytochemical studies. In the GEPR-9s, a larger number of GAD67 cRNA labeled neurons was observed in the ICCN as compared to SD rats. The external nucleus of the inferior colliculus was also found to contain significantly greater numbers of GAD67 cRNA labeled neurons whereas in the frontal cortex, a region of the brain that is not required for audiogenic seizure activity in GEPR-9s, there were no significant differences in hybridization between GEPR-9s and SD rats. Interestingly, within the superficial layers of the superior colliculus there was a higher density of hybridization in GEPR-9s than in SD rats indicating higher levels of GAD expression.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
C E Ribak; J C Lauterborn; M S Navetta; C M Gall |
Related Documents
:
|
3251598 - A comparison of the effects of midazolam and pentobarbital on the dose-response of gaba... 3131588 - Use of l-glutamine in total parenteral nutrition. 10943728 - Striatal a2a adenosine receptor antagonism differentially modifies striatal glutamate o... 8515848 - Gaba and potassium effects on corticospinal and primary afferent tracts of neonatal rat... 8632208 - Fermentable carbohydrates elevate plasma enteroglucagon but high viscosity is also nece... 2869898 - Hexavalent chromium effects on motor activity and some metabolic aspects of wistar albi... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Epilepsy research Volume: 14 ISSN: 0920-1211 ISO Abbreviation: Epilepsy Res. Publication Date: 1993 Feb |
Date Detail:
|
Created Date: 1993-04-21 Completed Date: 1993-04-21 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8703089 Medline TA: Epilepsy Res Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 105-13 Citation Subset: IM |
Affiliation:
|
Department of Anatomy and Neurobiology, University of California, Irvine 92717. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autoradiography Epilepsy / enzymology*, genetics Glutamate Decarboxylase / metabolism* In Situ Hybridization Inferior Colliculi / enzymology* Neurons / enzymology RNA Probes RNA, Messenger / analysis* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
|
EY 07193/EY/NEI NIH HHS; NS 15669/NS/NINDS NIH HHS; NS 21377/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA Probes; 0/RNA, Messenger; EC 4.1.1.15/Glutamate Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of chronic aminophylline on the anticonvulsant efficacy of phenobarbital and valproate in ...
Next Document: Reduced susceptibility to seizures in carbonic anhydrase II deficient mutant mice.