Document Detail

The induction of protrusion by neomycin in human glioma cells is correlated with a decrease followed by an increase in filamentous actin.
MedLine Citation:
PMID:  7550074     Owner:  NLM     Status:  MEDLINE    
In this paper we describe an experimental investigation of the mechanism of motility of vertebrate cells. Human glioma cells were treated with neomycin, an inhibitor of the phosphatidylinositol cycle; and changes in cell motility and the cytoskeleton were examined by video, fluorescence, and scanning electron microscopy and by cytofluorometry. Neomycin stimulates a single protrusion of lamellipodia from the cell margin, which is correlated with an initial rapid decrease in the amount of F-actin throughout the cell, especially at the cell edge; the fragmentation of actin filaments within the lamellipodia; and the subsequent de novo polymerization of F-actin in a marginal band at the leading edge of lamellipodia. Changes in F-actin are paralleled by changes in the distribution and amount of gelsolin. These results support the hypothesis that protrusion is initiated by the gelsolin-mediated severing and subsequent depolymerization of cortical actin filaments, which weakens the cell cortex, allowing hydrostatic or gel osmotic pressure to force the cell margin to protrude. The accompanying polymerization of filaments actin at the leading edge of the protrusion may stabilize the protrusion and support its expansion.
B Safiejko-Mroczka; P B Bell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  19     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-11-09     Completed Date:  1995-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  655-74     Citation Subset:  IM    
Department of Zoology, University of Oklahoma, Norman 73019, USA.
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MeSH Terms
Actins / drug effects,  metabolism*
Cell Line
Cell Movement / drug effects
Cytoskeleton / drug effects*,  physiology,  ultrastructure
Flow Cytometry
Freeze Drying
Microscopy, Electron, Scanning
Microscopy, Video
Neomycin / pharmacology*
Phosphatidylinositols / metabolism*
Time Factors
Tumor Cells, Cultured
Reg. No./Substance:
0/Actins; 0/Phosphatidylinositols; 1404-04-2/Neomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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