Document Detail

The induction of growth arrest in fibroblasts by SV40 T antigen.
MedLine Citation:
PMID:  16850187     Owner:  NLM     Status:  MEDLINE    
DNA tumor viruses such as SV40, Ras and papillomaviruses are the most commonly used agents in immortalization of non-hematopoietic cells, but the results are quite different. Some of them even lead instead to a senescence-like state. To verify the potential of SV40 T antigen-mediated immortalization or properties and functions of it to regulate cell growth, human dermal fibroblasts were cultured and then transfected with eukaryotic expressing plasmid psv3-neo which containing SV40 T DNA. We found that expression of oncogenic SV40 T in human dermal fibroblasts resulted in growth, arrest, earlier than the occurrence of control cell senescence, although telomerase was positive and cells grew faster than control ones in early stage following transfection. These observations suggest that SV40 T antigen can activate growth arrest in human dermal fibroblasts under normal growth condition instead of always prolonging the lifespan of fibroblasts. Moreover, high rate of cell division in early stage after transfection may be associated with the expression of telomerase activity.
Xinwen Wang; Yuan Liu; Rui Dong; Yan Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biology reports     Volume:  33     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-07-19     Completed Date:  2006-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  181-6     Citation Subset:  IM    
Department of Oral Histology and Pathology, Center for Tissue Engineering, Stomatological College, The Fourth Military Medical University, Xi'an 710032, China.
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MeSH Terms
Antigens, Polyomavirus Transforming / genetics,  physiology*
Cell Division
Fibroblasts / cytology*
Skin / cytology
Telomerase / metabolism
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; EC

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