| The induction of autophagy by mechanical stress. | |
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MedLine Citation:
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PMID: 22024750 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability to respond and adapt to changes in the physical environment is a universal and essential cellular property. Here we demonstrated that cells respond to mechanical compressive stress by rapidly inducing autophagosome formation. We measured this response in both Dictyostelium and mammalian cells, indicating that this is an evolutionarily conserved, general response to mechanical stress. In Dictyostelium, the number of autophagosomes increased 20-fold within 10 min of 1 kPa pressure being applied and a similar response was seen in mammalian cells after 30 min. We showed in both cell types that autophagy is highly sensitive to changes in mechanical pressure and the response is graduated, with half-maximal responses at ~0.2 kPa, similar to other mechano-sensitive responses. We further showed that the mechanical induction of autophagy is TOR-independent and transient, lasting until the cells adapt to their new environment and recover their shape. The autophagic response is therefore part of an integrated response to mechanical challenge, allowing cells to cope with a continuously changing physical environment. |
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Authors:
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Jason S King; Douwe M Veltman; Robert H Insall |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Autophagy Volume: 7 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-04-02 Completed Date: 2012-06-18 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
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Languages: eng Pagination: 1490-9 Citation Subset: IM |
Affiliation:
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Beatson Institute for Cancer Research, Glasgow, Scotland, UK. J.King@Beatson.gla.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Animals Autophagy* Cell Line, Tumor Dictyostelium / cytology*, physiology Humans Phagosomes / metabolism Pressure Stress, Mechanical* TOR Serine-Threonine Kinases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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//Cancer Research UK |
| Chemical | |
Reg. No./Substance:
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EC 2.7.1.1/TOR Serine-Threonine Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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