| Ischemia/reperfusion-induced feline intestinal dysfunction: importance of granulocyte recruitment. | |
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MedLine Citation:
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PMID: 1323498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although previous studies have reported that neutrophils play an important role in mediating the microvascular injury observed after reperfusion of ischemic intestine, the contribution of these phagocytic cells to the mucosal dysfunction remains unclear. Three series of experiments consisting of an untreated group, a short-term monoclonal antibody (MAb) IB4 treatment group (MAb IB4 given on the day of the experiment), and a long-term MAb IB4 treatment group (3-day pretreatment with MAb IB4) were performed using autoperfused segments of cat ileum exposed to 3 hours of ischemia followed by 1 hour of reperfusion. Mucosal myeloperoxidase activity, an index of mucosal granulocyte levels, increased from 12 to 25 U/g wet wt in the untreated group. In the short-term MAb IB4 experiments, baseline values were very similar to those of the untreated group but no increase in myeloperoxidase activity was observed after ischemia/reperfusion. Long-term pretreatment with MAb IB4 reduced baseline values of myeloperoxidase activity to approximately 1 U/g wet wt; the values remained at this level throughout the experiment. The permeability of the mucosal barrier was quantitated by measuring blood-to-lumen clearance to 51Cr-ethyl-enediaminetetraacetic acid (EDTA). The water absorptive capacity of the intestine was also measured. In the untreated group, mucosal permeability to 51Cr-EDTA increased sixfold and water absorption was abolished after reperfusion. Both short-term and long-term administration of MAb IB4 prevented the net fluid loss into the lumen, but only long-term administration of MAb IB4 blunted the increased mucosal permeability induced by ischemia/reperfusion. These data suggest that interstitial granulocytes contribute significantly to the mucosal dysfunction associated with reperfusion of the ischemic intestine. |
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Authors:
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P Kubes; J Hunter; D N Granger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 103 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-09-15 Completed Date: 1992-09-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 807-12 Citation Subset: AIM; IM |
Affiliation:
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Department of Medical Physiology, University of Calgary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Cats Cell Membrane Permeability Chromium Radioisotopes / metabolism Edetic Acid / metabolism Granulocytes / physiology* Intestinal Absorption / physiology* Intestinal Mucosa / physiology* Ischemia / physiopathology* Peroxidase / antagonists & inhibitors, metabolism Reperfusion Injury / physiopathology* Water / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK 33594/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Chromium Radioisotopes; 60-00-4/Edetic Acid; 7732-18-5/Water; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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