Document Detail


Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines.
MedLine Citation:
PMID:  18652819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formation of the dorsal organizer (Spemann organizer) is an important process in early vertebrate development. In zebrafish, two molecular cascades--Bozozok/Dharma (Boz) and Nodal signaling--act in parallel to induce the dorsal organizer. However, the complete molecular mechanism regulating this event remains unclear. Here we report that zebrafish cell lines derived from various developmental stages can induce a secondary axis when they are implanted into the mid-blastula but not the early gastrula. The implanted cells themselves did not differentiate, but instead induced ectopic expression of dorsal organizer markers in cells around the implanted cells and induced notochord formation in the secondary axis. These results indicate that cultured cell lines have the ability to induce a secondary axis through the initiation of dorsal organizer activity. However, ectopic expression of boz and sqt were not observed in cultured cells. In addition, implanted cell lines could induce the dorsal organizer even in maternal-zygotic one-eyed pinhead mutants, which are not responsive to Nodal signaling. Finally, the Nodal signaling pathway was not activated following implantation of cultured cells. Collectively, these data suggest that zebrafish cell lines induce the dorsal organizer independent of the boz and Nodal signaling pathways.
Authors:
Megumi Hashiguchi; Minori Shinya; Mika Tokumoto; Noriyoshi Sakai
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-07-04
Journal Detail:
Title:  Developmental biology     Volume:  321     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-10-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  387-96     Citation Subset:  IM    
Affiliation:
Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima 411-8540, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning / physiology*
Cells, Cultured
Embryonic Induction / physiology*
Homeodomain Proteins / genetics,  metabolism*
In Situ Hybridization
Mutation / genetics
Nodal Protein
Organizers, Embryonic / embryology*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / physiology*
Transcription Factors / genetics
Transforming Growth Factor beta / metabolism*
Zebrafish / embryology*
Zebrafish Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Nodal Protein; 0/Transcription Factors; 0/Transforming Growth Factor beta; 0/Zebrafish Proteins; 0/dharma protein, zebrafish; 0/oep protein, zebrafish

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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