| An increase in the synthesis and release of calcitonin gene-related peptide in two-kidney, one-clip hypertensive rats. | |
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MedLine Citation:
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PMID: 12832107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous investigations have indicated that capsaicin-sensitive sensory nerves play an important role in modulation of the peripheral resistance of the circulation system. In the present study, we examined the role of capsaicin-sensitive sensory nerves in two-kidney, one-clip (2K1C) renovascular hypertension rats. Systolic blood pressure (BP) was monitored by the tail-cuff method throughout the experiment. Concentrations of calcitonin gene-related peptide (CGRP) in the plasma, the level of CGRP mRNA in dorsal root ganglia (DRG) and the density of CGRP immunoreactive (CGRP-ir) fibers in mesenteric artery were measured. Blood pressure was significantly elevated at day 10 postoperation (BP was 143+/-10 and 114+/-7 mm Hg for 2K1C and Sham groups, respectively, p<0.05). Treatment with capsaicin, which selectively depletes neurotransmitters in sensory nerves, enhanced hypertensive responses to clipping (BP was 168+/-7 and 143+/-10 mm Hg at day 10 postoperation for Cap1+2K1C and 2K1C groups, respectively, p<0.05), and BP in the rats treated with a second injection of capsaicin was greater than that in the rats treated with a single injection of capsaicin (At day 30 postoperation, BP was 199+/-7 and 166+/-9 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01; mean arterial pressure was 185.2+/-6.6 and 150.5+/-4.1 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01). The expression of alpha-CGRP mRNA in DRG (122.87+/-3.67 arbitrary units, p<0.05), the level of CGRP in the plasma (75.40+/-4.99 pg/ml, p<0.01) and the density of CGRP-ir fibers in mesenteric artery (525.67+/-31.42 intersections, p<0.05) were significantly increased in 2K1C rats. Treatment with capsaicin, a single injection or a second injection, prevented the increased in the expression of CGRP mRNA in DRG. However, the decreased level of CGRP was only observed in the rats treated with a second capsaicin. These results suggest that in 2K1C hypertensive rats, the activity of capsaicin-sensitive sensory nerves is increased, which is playing a compensatory depressor role to partially counteract the increase in blood pressure, and that the cardiovascular actions of CGRP is mediated by the alpha-CGRP isoform. |
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Authors:
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Pan-Yue Deng; Feng Ye; Hui-Qing Zhu; Wei-Jun Cai; Han-Wu Deng; Yuan-Jian Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Regulatory peptides Volume: 114 ISSN: 0167-0115 ISO Abbreviation: Regul. Pept. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-30 Completed Date: 2004-04-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100479 Medline TA: Regul Pept Country: Netherlands |
Other Details:
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Languages: eng Pagination: 175-82 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, No. 88, Xiang-Ya Road, Changsha, Hunan 410078, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Calcitonin Gene-Related Peptide / biosynthesis, blood, genetics, metabolism* DNA Primers Hypertension / metabolism* Immunohistochemistry Male Mesenteric Arteries / metabolism RNA, Messenger / genetics Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Messenger; 83652-28-2/Calcitonin Gene-Related Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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