Document Detail


An increase in the synthesis and release of calcitonin gene-related peptide in two-kidney, one-clip hypertensive rats.
MedLine Citation:
PMID:  12832107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous investigations have indicated that capsaicin-sensitive sensory nerves play an important role in modulation of the peripheral resistance of the circulation system. In the present study, we examined the role of capsaicin-sensitive sensory nerves in two-kidney, one-clip (2K1C) renovascular hypertension rats. Systolic blood pressure (BP) was monitored by the tail-cuff method throughout the experiment. Concentrations of calcitonin gene-related peptide (CGRP) in the plasma, the level of CGRP mRNA in dorsal root ganglia (DRG) and the density of CGRP immunoreactive (CGRP-ir) fibers in mesenteric artery were measured. Blood pressure was significantly elevated at day 10 postoperation (BP was 143+/-10 and 114+/-7 mm Hg for 2K1C and Sham groups, respectively, p<0.05). Treatment with capsaicin, which selectively depletes neurotransmitters in sensory nerves, enhanced hypertensive responses to clipping (BP was 168+/-7 and 143+/-10 mm Hg at day 10 postoperation for Cap1+2K1C and 2K1C groups, respectively, p<0.05), and BP in the rats treated with a second injection of capsaicin was greater than that in the rats treated with a single injection of capsaicin (At day 30 postoperation, BP was 199+/-7 and 166+/-9 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01; mean arterial pressure was 185.2+/-6.6 and 150.5+/-4.1 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01). The expression of alpha-CGRP mRNA in DRG (122.87+/-3.67 arbitrary units, p<0.05), the level of CGRP in the plasma (75.40+/-4.99 pg/ml, p<0.01) and the density of CGRP-ir fibers in mesenteric artery (525.67+/-31.42 intersections, p<0.05) were significantly increased in 2K1C rats. Treatment with capsaicin, a single injection or a second injection, prevented the increased in the expression of CGRP mRNA in DRG. However, the decreased level of CGRP was only observed in the rats treated with a second capsaicin. These results suggest that in 2K1C hypertensive rats, the activity of capsaicin-sensitive sensory nerves is increased, which is playing a compensatory depressor role to partially counteract the increase in blood pressure, and that the cardiovascular actions of CGRP is mediated by the alpha-CGRP isoform.
Authors:
Pan-Yue Deng; Feng Ye; Hui-Qing Zhu; Wei-Jun Cai; Han-Wu Deng; Yuan-Jian Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regulatory peptides     Volume:  114     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-30     Completed Date:  2004-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, No. 88, Xiang-Ya Road, Changsha, Hunan 410078, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Calcitonin Gene-Related Peptide / biosynthesis,  blood,  genetics,  metabolism*
DNA Primers
Hypertension / metabolism*
Immunohistochemistry
Male
Mesenteric Arteries / metabolism
RNA, Messenger / genetics
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Messenger; 83652-28-2/Calcitonin Gene-Related Peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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