Document Detail

The incorporation and utilization of radiolabelled lipids by adult Schistosoma mansoni in vitro.
MedLine Citation:
PMID:  7442709     Owner:  NLM     Status:  MEDLINE    
Live adult Schistosoma mansoni, maintained in vitro were able to absorb and utilize radiolabelled arachidonic acid, linolenic acid, 3-sn-phosphatidylcholine, tripalmitylglycerol and cholesterol in the culture medium. Differential centrifugation demonstrated that all these lipids were incorporated into the parasite's various membrane structures. Analysis by thin-layer chromatography of the labelled compounds which resulted from incubation with the labelled lipids revealed that arachidonic acid, linolenic acid and fatty acids of tripalmitylglycerol and 3-sn-phosphatidylcholine were present largely as triacylglycerol with smaller amounts of labelled diacylglycerol, phospholipids and fatty acids. The labelled polar head group of 3-sn-phosphatidylcholine was cleaved from the molecule during incorporation, which suggested that hydrolysis of complex lipids is an integral part of the absorption mechanism. Cholesterol was not apparently altered during incorporation or further metabolized. It was also observed that arachidonic acid was incorporated more readily than the other lipids, however, no prostaglandin biosynthesis was detected.
F D Rumjanek; A J Simpson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  1     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1981-02-24     Completed Date:  1981-02-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  31-44     Citation Subset:  IM    
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MeSH Terms
Arachidonic Acids / metabolism
Cholesterol / metabolism
Linolenic Acids / metabolism
Lipid Metabolism*
Membranes / metabolism
Palmitates / metabolism
Phosphatidylcholines / metabolism
Prostaglandins / biosynthesis
Schistosoma mansoni / metabolism*
Triglycerides / metabolism
Reg. No./Substance:
0/Arachidonic Acids; 0/Linolenic Acids; 0/Palmitates; 0/Phosphatidylcholines; 0/Prostaglandins; 0/Triglycerides; 555-44-2/tripalmitin; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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