Document Detail


The incorporation of poly(lactic-co-glycolic) acid nanoparticles into porcine small intestinal submucosa biomaterials.
MedLine Citation:
PMID:  18076986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Small intestinal submucosa (SIS) derived from porcine small intestine has been intensively studied for its capacity in repairing and regenerating wounded and dysfunctional tissues. However, SIS suffers from a large spectrum of heterogeneity in microarchitecture leading to inconsistent results. In this study, we introduced nanoparticles (NPs) to SIS with an intention of decreasing the heterogeneity and improving the consistency of this biomaterial. As determined by scanning electron microscopy and urea permeability, the optimum NP size was estimated to be between 200 nm and 500 nm using commercial monodisperse latex spheres. The concentration of NPs that is required to alter pore sizes of SIS as determined by urea permeability was estimated to be 1 mg/ml 260 nm poly(lactic-co-glycolic) acid (PLGA) NPs. The 1mg/ml PLGA NPs loaded in the SIS did not change the tensile properties of the unmodified SIS or even alter pH values in a cell culture environment. More importantly, PLGA NP modified SIS did not affect human mammary endothelial cells (HMEC-1) morphology or adhesion, but actually enhanced HEMC-1 cell growth.
Authors:
Fadee G Mondalek; Benjamin J Lawrence; Bradley P Kropp; Brian P Grady; Kar-Ming Fung; Sundar V Madihally; Hsueh-Kung Lin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-04-17     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1159-66     Citation Subset:  IM    
Affiliation:
Department of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK 73019, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry*
Biomechanics
Cell Line
Cell Proliferation
Endothelial Cells / cytology
Humans
Intestinal Mucosa / chemistry*,  ultrastructure
Intestine, Small / chemistry,  ultrastructure
Lactic Acid / chemistry*
Materials Testing
Microscopy, Electron, Scanning
Nanoparticles / chemistry,  ultrastructure
Particle Size
Permeability
Polyglycolic Acid / chemistry*
Polymers / chemistry*
Regeneration
Swine
Tissue Engineering / methods
Urea
Wound Healing
Grant Support
ID/Acronym/Agency:
1 R21 DK 074858-01A2/DK/NIDDK NIH HHS; R21 DK074858/DK/NIDDK NIH HHS; R21 DK074858-01A2/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 57-13-6/Urea
Comments/Corrections

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