Document Detail


The C3H/HeJ inbred mouse is a model of vesico-ureteric reflux with a susceptibility locus on chromosome 12.
MedLine Citation:
PMID:  20407478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vesico-ureteric reflux is the most common congenital anomaly of the urinary tract, characterized by a defective uretero-vesical junction with retrograde urine flow from the bladder toward the kidneys. Because there is strong evidence for a genetic basis for some cases of vesico-ureteric reflux, we screened 11 inbred mouse strains for reflux and kidney size and identified one strain, C3H/HeJ, that has a 100 percent incidence of vesico-ureteric reflux with otherwise normal kidneys at birth. These mice are predisposed to reflux as a result of a defective uretero-vesical junction characterized by a short intravesical ureter. This defect results from a delay in urinary tract development initially manifested by a ureteric bud arising from a more caudal location along the mesonephric duct. In contrast, C57BL/6J mice (resistant to reflux at birth) have long intravesical ureters, normally positioned ureteric buds, and no delay in urinary tract development. Genome-wide and additional fine mapping of backcross mice, derived from C3H/HeJ and C57BL/6J crosses, identified a significant reflux susceptibility locus, Vurm1, on chromosome 12 (peak logarithm of the odds=7.39). The C3H/HeJ mouse is a model of vesico-ureteric reflux without renal malformation, and further characterization of this model will allow for the identification of a pathway important for urinary tract development, a finding that will serve as a model for the human disorder.
Authors:
Inga J Murawski; Rita W Maina; Danielle Malo; Lisa M Guay-Woodford; Philippe Gros; Mary Fujiwara; Kenneth Morgan; Indra R Gupta
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-21
Journal Detail:
Title:  Kidney international     Volume:  78     ISSN:  1523-1755     ISO Abbreviation:  Kidney Int.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2011-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-78     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, McGill University, Research Institute of the McGill University Health Center, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes, Mammalian / genetics*
Crosses, Genetic
Disease Models, Animal*
Genetic Predisposition to Disease
Humans
Kidney / abnormalities
Male
Mice
Mice, Inbred C3H*
Mice, Inbred C57BL
Physical Chromosome Mapping
Ureter / abnormalities
Urinary Bladder / abnormalities
Urinary Tract / abnormalities,  embryology
Vesico-Ureteral Reflux / embryology,  genetics*
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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