| The in vivo and in vitro effects of an alkylating agent, mechlorethamine, on IL-6 production in mice and the role of macrophages. | |
| | |
MedLine Citation:
|
PMID: 8886850 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Alkylating agents, cyclophosphamide (CY) and the related compound mechlorethamine (NM), significantly increase in vivo the blood level of IL-6 but not of IL-1. Since in vitro CY is inactive we have used in our experiments NM, a compound structurally and functionally related to phosphoramide mustard, the natural biologically active metabolite of CY. Thioglycollate or oil-induced peritonal macrophages (Mf) of four different mouse strains treated with NM produce significantly more IL-6 than the non-treated cells. In contrast, under these conditions, the production of IL-1, TNF alpha and NO/NO2 radicals is not affected. The NM-induced elevated production of IL-6 by Mf could not be further increased by the treatment of cells with LPS, which may suggest that both agonists stimulate the same signalling pathway. The antineoplastic activity of the alkylating agents like CY or NM is usually attributed to the interstrand cross-linking of the DNA of the dividing cells. Our experiments can be tentatively interpreted as the induced overplus of IL-6 can also contribute to the stimulation of the cytotoxic NK-cells. |
| | |
Authors:
|
K Bryniarski; M Ptak; W Ptak |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Immunopharmacology Volume: 34 ISSN: 0162-3109 ISO Abbreviation: Immunopharmacology Publication Date: 1996 Sep |
Date Detail:
|
Created Date: 1997-01-23 Completed Date: 1997-01-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7902474 Medline TA: Immunopharmacology Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 73-8 Citation Subset: IM |
Affiliation:
|
Department of Immunology, Collegium Medicum, Jagiellonian University, Kraków, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkylating Agents
/
pharmacology* Animals Cyclophosphamide / pharmacology Interleukin-1 / biosynthesis Interleukin-6 / biosynthesis* Macrophages / drug effects*, metabolism Male Mechlorethamine / pharmacology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Tumor Necrosis Factor-alpha / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/Alkylating Agents; 0/Interleukin-1; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 50-18-0/Cyclophosphamide; 51-75-2/Mechlorethamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of co-stimulatory molecules by tumor cells decreases tumorigenicity but may also reduce s...
Next Document: Selective inhibition of the alternative complement pathway by sCR1[desLHR-A] protects the rabbit iso...