| An in vivo study of the effects of ischaemia on uterine contraction, intracellular pH and metabolites in the rat. | |
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MedLine Citation:
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PMID: 8046648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are no data concerning the functional or metabolic effects of hypoxia in vivo in smooth muscle. We have therefore used 31P-NMR spectroscopy and intra-uterine pressure measurements to examine simultaneously, in vivo, the effect of ischaemia on uterine metabolites, intracellular pH (pHi) and force. A 1-2 cm portion of uterus from day 1 postpartum anaesthetized rats was exteriorized and an NMR surface coil placed on it. A balloon catheter in the uterine lumen recorded intra-uterine pressure changes from the same area. Reversible occluders were placed around the uterine artery. Occlusion produced a decrease and then abolition of contractions, within 10 min. In four of five animals contraction was abolished within 2 min. Upon reperfusion force was rapidly restored (1 min), in all preparations. The mean level of force was significantly above control (pre-occlusion) 20-30 min after reperfusion. The NMR data showed a significant fall in [ATP] (28%) and [phosphocreatine] (34%) during occlusion. Inorganic phosphate doubled in concentration during this period. Metabolites recovered slowly upon reperfusion, taking 20-30 min to return to pre-occlusion levels. The mean pHi fell from 7.32 to 7.00 upon occlusion and was rapidly reversed upon reperfusion. The changes in pHi closely correlated with the changes in uterine force. Decreases of pHi of a similar magnitude in vitro have previously been shown to abolish contractions; thus it is suggested that during ischaemia in vivo the depression of contraction is caused by the large fall in pHi. |
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Authors:
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N Harrison; J B Larcombe-McDouall; L Earley; S Wray |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of physiology Volume: 476 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-09-01 Completed Date: 1994-09-01 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 349-54 Citation Subset: IM |
Affiliation:
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Physiological Laboratory, University of Liverpool. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / physiopathology Female Hydrogen-Ion Concentration Ischemia / physiopathology* Magnetic Resonance Spectroscopy Muscle, Smooth / blood supply, metabolism, physiology* Phosphates / metabolism Phosphocreatine / metabolism Rats Rats, Wistar Regional Blood Flow / physiology Uterine Contraction / physiology* Uterus / blood supply*, physiology |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Phosphates; 67-07-2/Phosphocreatine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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