Document Detail

An in vivo study of the effects of ischaemia on uterine contraction, intracellular pH and metabolites in the rat.
MedLine Citation:
PMID:  8046648     Owner:  NLM     Status:  MEDLINE    
There are no data concerning the functional or metabolic effects of hypoxia in vivo in smooth muscle. We have therefore used 31P-NMR spectroscopy and intra-uterine pressure measurements to examine simultaneously, in vivo, the effect of ischaemia on uterine metabolites, intracellular pH (pHi) and force. A 1-2 cm portion of uterus from day 1 postpartum anaesthetized rats was exteriorized and an NMR surface coil placed on it. A balloon catheter in the uterine lumen recorded intra-uterine pressure changes from the same area. Reversible occluders were placed around the uterine artery. Occlusion produced a decrease and then abolition of contractions, within 10 min. In four of five animals contraction was abolished within 2 min. Upon reperfusion force was rapidly restored (1 min), in all preparations. The mean level of force was significantly above control (pre-occlusion) 20-30 min after reperfusion. The NMR data showed a significant fall in [ATP] (28%) and [phosphocreatine] (34%) during occlusion. Inorganic phosphate doubled in concentration during this period. Metabolites recovered slowly upon reperfusion, taking 20-30 min to return to pre-occlusion levels. The mean pHi fell from 7.32 to 7.00 upon occlusion and was rapidly reversed upon reperfusion. The changes in pHi closely correlated with the changes in uterine force. Decreases of pHi of a similar magnitude in vitro have previously been shown to abolish contractions; thus it is suggested that during ischaemia in vivo the depression of contraction is caused by the large fall in pHi.
N Harrison; J B Larcombe-McDouall; L Earley; S Wray
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  476     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-09-01     Completed Date:  1994-09-01     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Physiological Laboratory, University of Liverpool.
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MeSH Terms
Anoxia / physiopathology
Hydrogen-Ion Concentration
Ischemia / physiopathology*
Magnetic Resonance Spectroscopy
Muscle, Smooth / blood supply,  metabolism,  physiology*
Phosphates / metabolism
Phosphocreatine / metabolism
Rats, Wistar
Regional Blood Flow / physiology
Uterine Contraction / physiology*
Uterus / blood supply*,  physiology
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Phosphates; 67-07-2/Phosphocreatine

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