| An in vivo study of common carp (Cyprinus carpio L.) liver during prolonged hypoxia. | |
| | |
MedLine Citation:
|
PMID: 20483301 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Hypoxia induced apoptosis has been studied extensively in many mammalian cell lines but there are only a few studies using whole animal models. We investigated the response of the intact liver to hypoxia in a hypoxia tolerant fish, the carp (Cyprinus carpio, L). We exposed carp to hypoxia for up to 42 days, using oxygen level (0.5 mgO(2)/L) that were slightly higher than the critical oxygen level of carp. There was extensive DNA damage in liver cells, especially during the first week of exposure, indicated by a massive TUNEL signal. However there was no change in cell proliferation, cell number or size, no increase in caspase-3 activity, no increase in single stranded DNA and this, combined with a number of other observations, led us to conclude there was no increase in apoptosis in the liver during hypoxia. There was up-regulation of some anti-apoptotic genes and proteins (Bcl-2, HSP70, p27) and down-regulation of some pro-apoptotic genes (Tetraspanin 5 and Cell death activator). The cells appeared to enter cell cycle arrest, presumably to allow repair of damaged DNA. As there was no change in cell proliferation and cell number, the damaged cells were not entering apoptosis and must have recovered during prolonged hypoxia. |
| | |
Authors:
|
W L Poon; C Y Hung; K Nakano; D J Randall |
Publication Detail:
|
Type: Journal Article Date: 2007-05-18 |
Journal Detail:
|
Title: Comparative biochemistry and physiology. Part D, Genomics & proteomics Volume: 2 ISSN: 1878-0407 ISO Abbreviation: Comp. Biochem. Physiol. Part D Genomics Proteomics Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2010-05-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101270611 Medline TA: Comp Biochem Physiol Part D Genomics Proteomics Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 295-302 Citation Subset: - |
Affiliation:
|
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, S.A.R., China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evidence for a carbonic anhydrase-related protein in the brain of rainbow trout (Oncorhynchus mykiss...
Next Document: Gene expression in the liver of rainbow trout, Oncorhynchus mykiss, during the stress response.