| An in vivo evaluation of Brilliant Blue G in animals and humans. | |
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MedLine Citation:
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PMID: 18653608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: To evaluate the retinal toxicity of Brilliant Blue G (BBG) following intravitreal injection in rat eyes and examine the biocompatibility and the staining properties in humans. METHODS: BBG was injected into the 11 rat eyes to evaluate toxic effects with balanced salt solution (BSS) serving as control. Retinal toxicity was assessed by retinal ganglion cell (RGC) counts and by light microscopy 7 days later. In addition, BBG was applied during vitrectomy for macular hole (MH) (n = 15) or epiretinal membranes (ERM) (n = 3) in a prospective, non-comparative consecutive series of patients. Before and after surgery, all patients underwent a complete clinical examination including measurement of best corrected visual acuity (VA) and intraocular pressure, perimetry, fundus photography and optical coherence tomography. Patients were seen 1 day before surgery and then in approximately four weeks intervals. RESULTS: No significant reduction in RGC numbers and no morphological alterations were noted. A sufficient staining of the internal limiting membrane (ILM) was seen in patients with MH, while the staining pattern in ERM cases was patchy, indicating that parts of the ILM were peeled off along with the ERM in a variable extent. All MHs could be closed successfully. VA improved in 10 eyes (56%; 8/15 MH patients, 2/3 ERM patients), was unchanged in four eyes (22%; all MH patients) and was reduced in four eyes (22%; 3/15 MH, 1/3 ERM). No toxic effects attributable to the dye were noted during patient follow-up. The ultrastructure of tissue harvested during surgery was unremarkable. CONCLUSION: Brilliant Blue provides a sufficient and selective staining of the ILM. No retinal toxicity or adverse effects related to the dye were observed in animal and human studies. The long-term safety of this novel dye will have to be evaluated in larger patient series and a longer follow-up. |
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Authors:
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M Remy; S Thaler; R G Schumann; C A May; M Fiedorowicz; F Schuettauf; M Grüterich; S G Priglinger; M M Nentwich; A Kampik; C Haritoglou |
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Publication Detail:
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Type: Clinical Trial; Journal Article |
Journal Detail:
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Title: The British journal of ophthalmology Volume: 92 ISSN: 1468-2079 ISO Abbreviation: Br J Ophthalmol Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-25 Completed Date: 2008-08-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421041 Medline TA: Br J Ophthalmol Country: England |
Other Details:
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Languages: eng Pagination: 1142-7 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Animals Benzenesulfonates / diagnostic use, toxicity* Cell Count Coloring Agents / toxicity* Epiretinal Membrane / diagnosis, pathology, surgery Female Humans Male Middle Aged Prospective Studies Rats Rats, Inbred BN Retina / drug effects*, pathology, ultrastructure Retinal Ganglion Cells / drug effects, pathology Retinal Perforations / surgery Staining and Labeling / methods Vitrectomy / methods |
| Chemical | |
Reg. No./Substance:
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0/Benzenesulfonates; 0/Coloring Agents; 25305-78-6/brilliant blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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