Document Detail

The in vivo effects of neutralizing antibodies against IFN-gamma, IL-4, or IL-10 on the humoral immune response in young and aged mice.
MedLine Citation:
PMID:  7720078     Owner:  NLM     Status:  MEDLINE    
In the present study we investigated whether age-related changes in the composition and functional properties of murine CD4+ T cells are reflected in vivo by a changed humoral response to influenza vaccine in aged mice. After the primary immunization, the titers of influenza-specific IgM, IgG1, IgG2a, and IgG2b, but not of IgG3 and IgE, were significantly reduced in aged mice compared to young mice. Treatment of aged mice with anti-IFN-gamma, anti-IL-4, or anti-IL-10 resulted in levels of IgM and IgG1 comparable to those found in young mice, whereas IgG2a and IgG2b were further decreased. After the booster immunization IgE was significantly enhanced in aged mice, whereas no differences were observed with regard to the other isotypes. During the primary response in young mice, anti-IFN-gamma stimulated IgG1 and IgE, whereas an inhibition of IgG2a, IgG2b, and IgG3 was observed. Anti-IL-4 caused a decrease only in IgG3 while anti-IL-10 increased IgM and IgG1 and decreased IgG2b and IgG3. During the primary response in aged mice, all anti-cytokine antibodies enhanced IgM and IgG1 while IgE was only enhanced by anti-IL-10. By contrast, IgG3 was inhibited by anti-IFN-gamma and anti-IL-10. Anti-cytokine treatment of young mice increased all isotypes, except IgG3, in the secondary response, whereas the secondary response in aged mice was largely insensitive to anti-cytokine treatment. These data therefore support the idea that the in vivo effects of cytokines on isotype switching are dependent on the differentiation stage of B cells which may be different in young and aged mice.
R Dobber; M Tielemans; L Nagelkerken
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cellular immunology     Volume:  160     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-05-22     Completed Date:  1995-05-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Division Immunological and Infectious Diseases, TNO Prevention and Health, Leiden, The Netherlands.
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MeSH Terms
Aging / immunology*
Antibodies, Monoclonal / immunology,  pharmacology*
Antibodies, Viral / biosynthesis
Antibody Formation / drug effects*
CD4-Positive T-Lymphocytes / immunology*
Hemagglutinin Glycoproteins, Influenza Virus
Hemagglutinins, Viral / immunology
Immunization, Secondary
Immunoglobulin Class Switching / physiology*
Immunoglobulin E / biosynthesis
Immunoglobulin G / biosynthesis
Immunoglobulin M / biosynthesis
Influenza A virus / immunology
Influenza Vaccines / immunology
Interferon-gamma / antagonists & inhibitors*,  immunology,  physiology
Interleukin-10 / antagonists & inhibitors*,  immunology,  physiology
Interleukin-4 / antagonists & inhibitors*,  immunology,  physiology
Mice, Inbred CBA
Neuraminidase / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Hemagglutinins, Viral; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Influenza Vaccines; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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