| The in vivo Pig-a gene mutation assay is useful for evaluating the genotoxicity of ionizing radiation in mice. | |
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MedLine Citation:
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PMID: 22911630 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The in vivo Pig-a mutation assay has been adapted for measuring mutation in rats, mice, monkeys, and humans. To date, the assay has been used mainly to assess the mutagenicity of chemicals that are known to be powerful point mutagens. The assay has not been used to measure the biological effects associated with ionizing radiation. In this study, we modified the Pig-a gene mutation assay (Kimoto et al. [2011b]: Mutat Res 723:36-42) and used 3-color staining with fluorescently labeled anti-CD24, anti-TER-119, and anti-CD71 to detect the Pig-a mutant frequencies in total red blood cells (RBCs) and in reticulocytes (RETs) from X-irradiated mice. Single exposures to X-irradiation resulted in dose- and time-dependent increases in Pig-a mutant frequencies, and these subsequently declined over time returning to background frequencies. The same total amount of radiation, delivered either as a single dose or as four repeat doses at weekly intervals, increased Pig-a mutant frequencies to comparable levels, reaching maxima 2-3 weeks after the single dose or 2-3 weeks after the last of the repeat doses. These increased frequencies subsequently returned to background levels. Our results indicated that the 3-color Pig-a assay was useful for evaluating the in vivo genotoxicity of radiation. Environ. Mol. Mutagen., 2012. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Shin Ohtani; Aiko Unno; Akira Ushiyama; Takafumi Kimoto; Daishiro Miura; Naoki Kunugita |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-22 |
Journal Detail:
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Title: Environmental and molecular mutagenesis Volume: - ISSN: 1098-2280 ISO Abbreviation: Environ. Mol. Mutagen. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8800109 Medline TA: Environ Mol Mutagen Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Environmental Health, National Institute of Public Health, Saitama, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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