| The in vitro and in vivo apoptotic effects of Mahonia oiwakensis on human lung cancer cells. | |
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MedLine Citation:
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PMID: 19497414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Both the root and stem bark of Mahonia species were popular folk medicines. The plant has several proven biological activities including anti-bacterial, anti-fungal, and anti-inflammatory effects. However, Mahonia has not been studied for its anticancer effects. In the present study, we made extracts from Mahonia oiwakensis (MOE), a selected species in Taiwan, and investigated their effects on various human lung cells. We found that MOE-induced apoptotic death in human A549 non-small-cell lung carcinoma (NSCLC) cells in a dose- and time-dependent manner. Treatment with the extracts also caused an increase in the sub-G1 fraction of cells, chromosome condensation, and DNA fragmentation. The mitochondrial-mediated pathway was implicated in this MOE-induced apoptosis as evidenced by the activation of the caspase cascade, cleavage of poly (ADP-ribose) polymerase (PARP), disruption of mitochondrial membrane potential, and release of cytochrome C. A higher ratio of Bax/Bcl-2 proteins and cleavage of Bid were also observed in MOE-induced cell apoptosis. In A549 tumor-xenografted nude mice, MOE also retarded in vivo proliferation (P<0.05) and induced apoptosis in tumor cells, as shown by a decrease in Ki-67-positive staining (P<0.05) and increased transferase-mediated dUTP nick-end labeling (TUNEL)-positive staining (P<0.05). In conclusion, MOE inhibits the growth of human lung cancer cells in vitro and in vivo, suggesting that it may have therapeutic potential against human lung cancer. |
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Authors:
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Bing-Sang Wong; Yung-Chin Hsiao; Ta-Wei Lin; Kuo-Shuen Chen; Pei-Ni Chen; Wu-Hsien Kuo; Shu-Chen Chu; Yih-Shou Hsieh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-05 |
Journal Detail:
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Title: Chemico-biological interactions Volume: 180 ISSN: 1872-7786 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-05 Completed Date: 2009-06-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: Ireland |
Other Details:
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Languages: eng Pagination: 165-74 Citation Subset: IM |
Affiliation:
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Division of Neurosurgery, Antai Tian-Sheng Memorial Hospital, Pingtung County, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / drug effects Cell Line, Tumor Cell Survival Dose-Response Relationship, Drug Humans Lung Neoplasms / classification, drug therapy* Mahonia / chemistry* Mice Mice, Nude Neoplasms, Experimental / drug therapy Plant Extracts / chemistry*, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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