Document Detail


The in vitro and in vivo apoptotic effects of Mahonia oiwakensis on human lung cancer cells.
MedLine Citation:
PMID:  19497414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both the root and stem bark of Mahonia species were popular folk medicines. The plant has several proven biological activities including anti-bacterial, anti-fungal, and anti-inflammatory effects. However, Mahonia has not been studied for its anticancer effects. In the present study, we made extracts from Mahonia oiwakensis (MOE), a selected species in Taiwan, and investigated their effects on various human lung cells. We found that MOE-induced apoptotic death in human A549 non-small-cell lung carcinoma (NSCLC) cells in a dose- and time-dependent manner. Treatment with the extracts also caused an increase in the sub-G1 fraction of cells, chromosome condensation, and DNA fragmentation. The mitochondrial-mediated pathway was implicated in this MOE-induced apoptosis as evidenced by the activation of the caspase cascade, cleavage of poly (ADP-ribose) polymerase (PARP), disruption of mitochondrial membrane potential, and release of cytochrome C. A higher ratio of Bax/Bcl-2 proteins and cleavage of Bid were also observed in MOE-induced cell apoptosis. In A549 tumor-xenografted nude mice, MOE also retarded in vivo proliferation (P<0.05) and induced apoptosis in tumor cells, as shown by a decrease in Ki-67-positive staining (P<0.05) and increased transferase-mediated dUTP nick-end labeling (TUNEL)-positive staining (P<0.05). In conclusion, MOE inhibits the growth of human lung cancer cells in vitro and in vivo, suggesting that it may have therapeutic potential against human lung cancer.
Authors:
Bing-Sang Wong; Yung-Chin Hsiao; Ta-Wei Lin; Kuo-Shuen Chen; Pei-Ni Chen; Wu-Hsien Kuo; Shu-Chen Chu; Yih-Shou Hsieh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-05
Journal Detail:
Title:  Chemico-biological interactions     Volume:  180     ISSN:  1872-7786     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-05     Completed Date:  2009-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  165-74     Citation Subset:  IM    
Affiliation:
Division of Neurosurgery, Antai Tian-Sheng Memorial Hospital, Pingtung County, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival
Dose-Response Relationship, Drug
Humans
Lung Neoplasms / classification,  drug therapy*
Mahonia / chemistry*
Mice
Mice, Nude
Neoplasms, Experimental / drug therapy
Plant Extracts / chemistry*,  pharmacology*
Chemical
Reg. No./Substance:
0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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