| The in vitro sensitivity of chronically hypoxic EMT6/SF cells to X-radiation and hypoxic cell radiosensitizers. | |
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MedLine Citation:
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PMID: 3159699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the effect of extreme, prolonged hypoxia on the radiosensitivity of EMT6/SF cells in vitro. As cells were kept hypoxic for 1-24 h, their radiosensitivity increased, but no further change was noted for hypoxic incubation beyond 24 h. Chronically hypoxic (45 h) cells were more radiosensitive than acutely hypoxic (1 h) cells by a factor of 1.43. When chronically hypoxic cells were re-aerated, the increased radiosensitivity persisted, although it was reduced. Misonidazole (MISO) radiosensitization was equally effective under conditions of acute and chronic hypoxia. In contrast, MISO, SR2555 and SR2508 were more cytotoxic in chronically hypoxic cultures than in acutely hypoxic cells. Measurements suggested that intracellular thiols may play an important role in the effects observed. |
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Authors:
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D C Shrieve; J W Harris |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology and related studies in physics, chemistry, and medicine Volume: 48 ISSN: 0020-7616 ISO Abbreviation: Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. Publication Date: 1985 Jul |
Date Detail:
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Created Date: 1985-08-12 Completed Date: 1985-08-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374725 Medline TA: Int J Radiat Biol Relat Stud Phys Chem Med Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 127-38 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Survival Cells, Cultured Etanidazole Female Mammary Neoplasms, Experimental / pathology* Mice Misonidazole / pharmacology Nitroimidazoles / pharmacology Oxygen / physiology* Radiation Tolerance* Radiation-Sensitizing Agents / pharmacology* Rats Sulfhydryl Compounds / pharmacology X-Rays |
| Chemical | |
Reg. No./Substance:
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0/Nitroimidazoles; 0/Radiation-Sensitizing Agents; 0/Sulfhydryl Compounds; 13551-87-6/Misonidazole; 22668-01-5/Etanidazole; 74141-74-5/SR 2555; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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