Document Detail


An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells.
MedLine Citation:
PMID:  20226236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asthma is a public health problem worldwide, and indoor air pollution considered to be a potential etiology. New tools need to be developed to study the effects of air pollutants in vitro and modelize inhalation exposure. This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells, and apply this to gaseous formaldehyde (FA). A549 cells were exposed using the direct exposure device (air/liquid interface) to FA without, after and before TNFalpha (1 ng/mL) sensitization. 24h after exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed. No effects on cellular viability were observed for concentrations < or =50 microg/m(3). After TNFalpha sensitization, FA-exposure induced a significant increase in IL-8 (p<0.001), which could lead to the initiation or pathogenesis of non-specific respiratory inflammation. The results of this study demonstrate the feasibility and sensitivity of the exposure system for testing inflammatory cellular effects of indoor gaseous compounds at environmental doses directly on human respiratory cells.
Authors:
Charles Persoz; Sophie Achard; Christopher Leleu; Isabelle Momas; Nathalie Seta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-10
Journal Detail:
Title:  Toxicology letters     Volume:  195     ISSN:  1879-3169     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-05-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Universit? Paris Descartes, Laboratoire de Sant? Publique et Environnement - EA 4064, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Survival
Chemokine CCL2 / metabolism
Environmental Exposure / analysis*
Epithelial Cells / drug effects*,  metabolism
Formaldehyde / toxicity*
Inflammation Mediators / metabolism*
Interleukin-6 / metabolism
Interleukin-8 / metabolism
Lung / drug effects*,  metabolism
Models, Biological
Tumor Necrosis Factor-alpha / metabolism
Volatile Organic Compounds / toxicity
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Inflammation Mediators; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 0/Volatile Organic Compounds; 50-00-0/Formaldehyde

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