| An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 20226236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Asthma is a public health problem worldwide, and indoor air pollution considered to be a potential etiology. New tools need to be developed to study the effects of air pollutants in vitro and modelize inhalation exposure. This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells, and apply this to gaseous formaldehyde (FA). A549 cells were exposed using the direct exposure device (air/liquid interface) to FA without, after and before TNFalpha (1 ng/mL) sensitization. 24h after exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed. No effects on cellular viability were observed for concentrations < or =50 microg/m(3). After TNFalpha sensitization, FA-exposure induced a significant increase in IL-8 (p<0.001), which could lead to the initiation or pathogenesis of non-specific respiratory inflammation. The results of this study demonstrate the feasibility and sensitivity of the exposure system for testing inflammatory cellular effects of indoor gaseous compounds at environmental doses directly on human respiratory cells. |
| | |
Authors:
|
Charles Persoz; Sophie Achard; Christopher Leleu; Isabelle Momas; Nathalie Seta |
Related Documents
:
|
9010506 - Induction of apoptosis and cytokine gene expression in t-cell lines by sera of patients... 11472346 - Aberrant expression of caspase cascade regulatory genes in adult t-cell leukaemia: surv... 19464056 - Role of microrna-143 in fas-mediated apoptosis in human t-cell leukemia jurkat cells. 18364356 - Requirement of inositol 1,4,5-trisphosphate receptors for tumor-mediated lymphocyte apo... 14997036 - Cellular immune activation in gulf war veterans. 269356 - Focal infection in perspective. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-10 |
Journal Detail:
|
Title: Toxicology letters Volume: 195 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-03 Completed Date: 2010-05-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 99-105 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Universit? Paris Descartes, Laboratoire de Sant? Publique et Environnement - EA 4064, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Cell Survival Chemokine CCL2 / metabolism Environmental Exposure / analysis* Epithelial Cells / drug effects*, metabolism Formaldehyde / toxicity* Inflammation Mediators / metabolism* Interleukin-6 / metabolism Interleukin-8 / metabolism Lung / drug effects*, metabolism Models, Biological Tumor Necrosis Factor-alpha / metabolism Volatile Organic Compounds / toxicity |
| Chemical | |
Reg. No./Substance:
|
0/Chemokine CCL2; 0/Inflammation Mediators; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 0/Volatile Organic Compounds; 50-00-0/Formaldehyde |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pathways involved in the generation of reactive oxygen and nitrogen species during glucose deprivati...
Next Document: NAD(+) and metabolic regulation of age-related proteoxicity: a possible role for methylglyoxal?'