Document Detail

The in vitro micronucleus assay for detection of cytogenetic effects induced by mutagen-carcinogens: comparison with the in vitro sister-chromatid exchange assay.
MedLine Citation:
PMID:  6749166     Owner:  NLM     Status:  MEDLINE    
The sensitivity of a cytogenetic assay, as expressed by the in vitro induction of micronuclei (MN), was compared to the in vitro induction of sister-chromatid exchanges (SCEs). Chinese hamster lung (V79) cells were exposed to 3 known alkylating agents: methyl methanesulphonate (MMS), ethyl methanesulphonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and to 5 newly synthesized naphthofurans: 2-nitro-7-methoxynaphtho[2,1-b]furan (A), 2-nitro-8-methoxynaphtho[2,1-b]furan (B), 2-nitronaphtho[2,1-b]furan (C), 2-nitro-7-bromonaphtho[2,1-b]furan (D) and 7-methoxynaphtho[2,1-b]furan (E). The induction of MN only was also analysed after exposure of the cells to 4 alcohols: ethanol, methanol, butanol and propanol. The lowest dose at which a significant effect could be observed was determined. In both assays, MNNG, MMS and EMS were equally active with the following order of potency: MNNG greater than MMS greater than EMS, the latter being a very weak inducer of MN and SCE. Compounds A and B were also very effective in both assays. Compound C was a more active inducer of SCE than MN. Compounds D and E were not active in either assay. None of the 4 alcohols induced MN. Our results are compared with the previously published data on in vitro and in vivo induction of SCE and MN. We conclude that the MN in vitro assay which detects clastogens as well as agents affecting the spindle apparatus, is a good indicator of genotoxicity, though slightly less sensitive than the in vitro SCE test. It could provide a rapid, simple and inexpensive complementary short-term test for the evaluation of potentially mutagenic chemicals.
C Lasne; Z W Gu; W Venegas; I Chouroulinkov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  130     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-09-13     Completed Date:  1984-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  273-82     Citation Subset:  IM    
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MeSH Terms
Alcohols / pharmacology
Alkylating Agents / pharmacology
Carcinogens / pharmacology*
Cell Nucleus / drug effects*
Cells, Cultured
Crossing Over, Genetic / drug effects*
Mutagenicity Tests*
Nitrofurans / pharmacology
Sister Chromatid Exchange / drug effects*
Reg. No./Substance:
0/Alcohols; 0/Alkylating Agents; 0/Carcinogens; 0/Nitrofurans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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