Document Detail


An in vitro investigation of aorta and corpus cavernosum from eNOS and nNOS gene-deficient mice.
MedLine Citation:
PMID:  14722775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to ascertain the relative contribution of the endothelial and neuronal nitric oxide (NO) synthase isoforms on NO-dependent vascular and nerve function in vitro, aorta and corpus cavernosum from mice deficient in their expression (eNOS-/- and nNOS-/-) were isolated in organ baths for tension measurements. Agonist or electrical field stimulation (EFS) evoked nerve-mediated responses were compared against wild-type controls. In aortas from nNOS-/- mice, contraction responses to phenylephrine were increased. Conversely, endothelium-dependent relaxation (EDR) to acetylcholine (ACh) was decreased. In contrast, eNOS-/- aortas showed decreased sensitivity to phenylephrine and developed a flurbiprofen-sensitive contraction to ACh, and sensitivity to the NO-donor sodium nitroprusside was increased. In cavernosum from eNOS-/- and nNOS-/- mice, maximum contractions to phenylephrine and EFS, and relaxation responses to nitroprusside, were increased. As in aorta, ACh addition led to a contractile response in eNOS-/- cavernosum. Maximum EFS induced non-adrenergic, non-cholinergic (NANC) nerve-mediated relaxation was increased in eNOS-/-, whilst being decreased in nNOS-/- cavernosum. These data suggest that whilst NO-dependent vascular function is primarily eNOS mediated, and nerve function nNOS mediated, aorta function may be at least partially reliant on nNOS-related mechanisms. In addition, mechanisms of physiological compensation were observed, which require further study.
Authors:
M R Nangle; M A Cotter; N E Cameron
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-01-13
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  448     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-22     Completed Date:  2004-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, AB25 2ZD, Aberdeen, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Animals
Aorta, Thoracic / drug effects,  physiology*
Body Weight / genetics
Dose-Response Relationship, Drug
Endothelium, Vascular / physiology
Enzyme Inhibitors / pharmacology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle Contraction / drug effects,  physiology
Muscle, Smooth, Vascular / drug effects,  innervation,  physiology*
Nitric Oxide Donors / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors,  deficiency,  genetics*
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Nitroarginine / pharmacology
Nitroprusside / pharmacology
Organ Size / drug effects
Penis / physiology*
Phenylephrine / pharmacology
Vasoconstrictor Agents / pharmacology
Vasodilator Agents / pharmacology
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Nitric Oxide Donors; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 15078-28-1/Nitroprusside; 2149-70-4/Nitroarginine; 51-84-3/Acetylcholine; 59-42-7/Phenylephrine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos1 protein, mouse; EC 1.14.13.39/Nos3 protein, mouse

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