Document Detail


An in vitro, ex vivo, and in vivo demonstration of the lipolytic effect of slimming liposomes: An unexpected alpha(2)-adrenergic antagonism.
MedLine Citation:
PMID:  12219247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most of the slimming products already developed for cosmetic applications did not result from strategies that integrate whole lipolysis-regulating mechanisms. We thus focused our attention on a more complete integration of these mechanisms and we developed slimming liposomes (SLC) containing two micro-circulation activators, i.e., esculoside and Centella asiatica extracts, one phosphodiesterase inhibitor, i.e., caffeine, and one fatty acid-beta oxidation activator, i.e., L-carnitine. The validity of our approach was assessed through (a) in vitro tests demonstrating that SLC induced a dramatic increase in the cyclic adenosine monophosphate (cAMP) content in human adipocytes, with a subsequent rise in the nonesterified fatty acids (NEFA) content of human adipocyte incubation medium, and (b) in vivo studies showing that SLC could provide an actual potent slimming effect on human volunteers. Moreover, we give here, through binding experiments, the unambiguous demonstration that SLC is able to antagonize the alpha(2)-adrenergic receptor that is known to reduce intracellular AMPc content and, subsequently, to down-regulate lipolysis. This alpha(2)-adrenergic antagonism has never been reported for any component of SLC, and this work is the first demonstration of the alpha(2)-adrenergic antagonism of such a combination of active liposome compounds.
Authors:
L Tholon; G Neliat; C Chesne; D Saboureau; E Perrier; J-E Branka
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; In Vitro; Journal Article    
Journal Detail:
Title:  Journal of cosmetic science     Volume:  53     ISSN:  1525-7886     ISO Abbreviation:  J Cosmet Sci     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2002-09-09     Completed Date:  2002-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9814276     Medline TA:  J Cosmet Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-18     Citation Subset:  IM    
Affiliation:
Coletica, 32 rue Saint Jean de Dieu, 69007 Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / drug effects,  metabolism
Adrenergic alpha-Antagonists / pharmacology
Cyclic AMP / metabolism
Lipolysis*
Liposomes*
Receptors, Adrenergic, alpha-2 / antagonists & inhibitors*
Weight Loss / drug effects*
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Antagonists; 0/Liposomes; 0/Receptors, Adrenergic, alpha-2; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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