Document Detail


An in vitro evaluation of the antibacterial efficacy of chlorine dioxide on E. faecalis in bovine incisors.
MedLine Citation:
PMID:  16123704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated the ability of chlorine dioxide to eliminate Enterococcus faecalis from dentinal tubules of bovine incisors. Thirty-seven extracted bovine incisor roots were sectioned into seventy-four 5 mm disks. Standardized lumens were filled with either sterile Brain Heart Infusion Broth (contamination controls, n = 10) or BHI containing E. faecalis (1.0 x 10 cfu/ml). Disks were incubated in 5% CO2 at 37 degrees C for 72 h. To simulate endodontic instrumentation the lumens were again enlarged. Sixty disks were randomly divided into four experimental groups and filled with one of the following irrigants: 10% Clidox-S (chlorine dioxide), 13.8% BioClenz (chlorine dioxide), 5.25% Clorox, or saline. The disks were incubated for 30 min and were then frozen, pulverized, serially diluted in phosphate buffered saline, and plated on BHI plates in triplicate. Total colony forming units were counted macroscopically. Statistical analysis of the data was performed with a Kruskal-Wallis one-way ANOVA on ranks (p < 0.05, n = 60). Bacterial counts, expressed in log10 cfu/disk were as follows (">" denotes significant differences): Saline > Clidox-S = BioClenz > Clorox. All negative controls were sterile. Chlorine dioxide and NaOCL were both effective in eliminating E. faecalis from the dentinal disks within 30 min.
Authors:
Russell S Eddy; Anthony P Joyce; Steven Roberts; Thomas B Buxton; Frederick Liewehr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  31     ISSN:  0099-2399     ISO Abbreviation:  J Endod     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-26     Completed Date:  2005-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  672-5     Citation Subset:  D    
Affiliation:
U.S. Army Endodontic Residency Program, Fort Gordon, Georgia, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Cattle
Chlorine Compounds / pharmacology*
Drug Evaluation, Preclinical
Enterococcus faecalis / drug effects*
Incisor
Oxides / pharmacology*
Random Allocation
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Chlorine Compounds; 0/Oxides; 10049-04-4/chlorine dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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