| The in vitro effects of proxymetacaine, fluorescein, and fusidic acid on real-time PCR assays used for the diagnosis of Feline herpesvirus 1 and Chlamydophila felis infections. | |
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MedLine Citation:
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PMID: 21923818 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Purpose To investigate the possible inhibition of qPCR assays used for the diagnosis of ocular infections in cats by proxymetacaine, fluorescein, and fusidic acid, which are commonly used in veterinary ophthalmology. Methods Fluorescein, proxymetacaine, and fusidic acid were tested for possible inhibition of a triplex qPCR assay designed to detect Chamydophila felis, Feline herpesvirus 1 (FHV-1), and the feline 28S ribosomal DNA (28S rDNA) gene by comparing threshold cycle (C(t) ) values of samples with and without the three products. A second experiment was carried out to measure the effects of various dilutions of fusidic acid. Results No statistically significant differences were detected between the C. felis, FHV-1, and 28S rDNA C(t) values with and without proxymetacaine or fluorescein. However, there was a statistically significant increase in FHV-1 (P < 0.01), C. felis (P < 0.01), and 28S rDNA (P < 0.05) C(t) values when fusidic acid was used. When dilutions of fusidic acid were tested, the results revealed that only the 1:2 dilution caused a statistically significant increase (P < 0.01) in the FHV-1 Ct values. Conclusion Proxymetacaine and fluorescein did not interfere with our qPCR assays for the detection of C. felis and FHV-1. The presence of fusidic acid caused a small inhibitory effect of doubtful clinical significance. In vivo studies are required to establish the clinical relevance of this study and to confirm our findings. |
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Authors:
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Sergi Segarra; Kostas Papasouliotis; Chris Helps |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Veterinary ophthalmology Volume: 14 Suppl 1 ISSN: 1463-5224 ISO Abbreviation: Vet Ophthalmol Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100887377 Medline TA: Vet Ophthalmol Country: England |
Other Details:
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Languages: eng Pagination: 5-8 Citation Subset: IM |
Copyright Information:
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© 2011 American College of Veterinary Ophthalmologists. |
Affiliation:
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School of Veterinary Sciences, Small Animal Hospital, University of Bristol, Bristol, UK School of Veterinary Sciences, Diagnostic Laboratories, Langford Veterinary Services, University of Bristol, Bristol, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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