| The in-vitro activity of povidone-iodinecream against Staphylococcus aureus and its bioavailability in nasal secretions. | |
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MedLine Citation:
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PMID: 10896798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Due to the emergence of mupirocin-resistance in some epidemic strains of methicillin resistant Staphylococcus aureus (EMRSA) and the appearance of EMRSA with intermediate resistance to vancomycin, we evaluated the in-vitro activity of 5% povidone-iodine ('Betadine') cream as a possiblealternative to mupirocin for the elimination of nasal carriage of S. aureus. As judged by enrichment culture, povidone-iodine was bactericidal against three mupirocin-sensitive strains of S. aureus from nasal carriers, and against mupirocin-resistant and -sensitive strains of EMRSA types 3, 15 and 16, after incubation with povidone-iodine for 1.0 min at 32 degrees C. Mupirocin nasal ointment did not prevent growth after 180 min incubation. In a quantitative suspension test, 1:100 dilution of povidone-iodine cream completely eliminated an inoculum of 10(8)cfu/mL of all nine test organisms after incubation at 32 degrees C for 1.0 min, and 1:1000 dilution reduced cfu, by a factor of 10(5). After direct inoculation of the povidone-iodine cream to give 10(5)cfu/g, none of the test strains were recoverable after 30 s, giving a killing rate of approximately 10(4)cfu/s; for mupirocin nasal ointment, the maximum reduction of mupirocin-sensitive strains was ten fold after 3 h. Povidone-iodine activity was not detectable in sensitivity-testing agar, although 0.025% of povidone-iodine was detectable in a 15% nutrient strength tryptone soya agar. Using this minimal medium, the addition of nasal secretions (from any of 11 samples) reduced the activity of povidone-iodine by 80-90%, but mupirocin activity was unaffected. One millilitre of nasal secretions inactivated the equivalent of approximately 22.5 mg of povidone-iodine. These results suggest that povidone-iodine cream may have a role in the prevention of colonization and infection caused by MRSA, including mupirocin-resistant strains. |
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Authors:
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R L Hill; M W Casewell |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of hospital infection Volume: 45 ISSN: 0195-6701 ISO Abbreviation: J. Hosp. Infect. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-08-15 Completed Date: 2000-08-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8007166 Medline TA: J Hosp Infect Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 198-205 Citation Subset: IM |
Affiliation:
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Dulwich Public Health Laboratory and Medical Microbiology, Guy's, King's and St Thomas' School of Medicine, King's Denmark Hill Campus, Bessemer Road, London, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology Anti-Infective Agents, Local / pharmacokinetics, pharmacology* Biological Availability Carrier State Drug Resistance, Microbial Humans Microbial Sensitivity Tests Mupirocin / pharmacology Nose / metabolism, microbiology Povidone-Iodine / pharmacokinetics, pharmacology* Staphylococcal Infections / prevention & control Staphylococcus aureus / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Anti-Infective Agents, Local; 12650-69-0/Mupirocin; 25655-41-8/Povidone-Iodine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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