Document Detail


The in vitro PDT efficacy of a novel metallophthalocyanine (MPc) derivative and established 5-ALA photosensitizing dyes against human metastatic melanoma cells.
MedLine Citation:
PMID:  21246581     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVE: Numerous worldwide clinical trials have shown that photodynamic therapy (PDT) represents an effective and safe modality for various skin disorders, but little research has been done in terms of its effect on malignant melanomas (MM). Thus, the aim of this study was to compare the effect of both established porphyrin photosensitizer 5-aminolevulinic acid (5-ALA) and novel metallophthalocyanine (MPc) photosensitizer on human metastatic skin cells which produce a MM.
MATERIALS AND METHODS: The cellular responses following PDT were assessed using changes in cell morphology, cell viability, cytotoxicity, apoptosis, and proliferation.
RESULTS: Findings reported that in vitro human MM cell line A375 (EACC no: 88113005) are highly sensitive to growth inhibition and apoptosis induction by the cytotoxic side-effects induced by MPc and 5-ALA photosensitizing treatments post-laser irradiation at 680 and 636 nm, respectively. The decrease of cell viability accompanied by an increased cytotoxicity and apoptotic and necrotic levels, with a time-dependant decrease in cellular proliferation was found to be far more significant for MPc-treated cells than 5-ALA-treated cells, since MPc was applied in far lower concentrations and exhibited far less photoxicity to control cells.
CONCLUSION: Hence, novel MPc proved to be the better photosensitizing dye for metastatic melanoma tumor destruction in combination with laser irradiation and is a particularly attractive photosensitizer since it exhibits so many ideal properties of a photosensitizing agent, thus further research of this possible anticancer agent could contribute to its potential application in PDT cancer treatment of MMs.
Authors:
C A Robertson; H Abrahamse; Denise Evans
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  42     ISSN:  1096-9101     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-06-28     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  766-76     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
Affiliation:
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Aminolevulinic Acid / pharmacology*
Cell Culture Techniques
Cell Line, Tumor / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Humans
Indoles / pharmacology*
Melanoma / drug therapy*,  secondary
Photochemotherapy*
Photosensitizing Agents / pharmacology*
Skin Neoplasms / drug therapy*,  pathology
Chemical
Reg. No./Substance:
0/Indoles; 0/Photosensitizing Agents; 106-60-5/Aminolevulinic Acid; 574-93-6/phthalocyanine
Comments/Corrections
Erratum In:
Lasers Surg Med. 2011 Sep;43(8):869
Note: Evans, Denise [added]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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