Document Detail


An improved technique for studying pleural fluid pressure and composition in rabbits.
MedLine Citation:
PMID:  9717066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Knowledge of pleural liquid pressure (Pliq) and composition is crucial for studies concerning intrapleural fluid dynamics, and pleural fluid turnover. We measured Pliq at intercostal and costal levels in anaesthetized spontaneously breathing rabbits using a minimally invasive method that assures a long-lasting hydraulic continuity between the pleural liquid and the recording system. Polyethylene tubes were glued either to the exposed endothoracic fascia or inserted into a rib to provide a scaled connection to the recording system. After inducing a pneumothorax with nitrous oxide (N2O) via an intrapleural cannula, a hole (approximately 0.7 mm2) was pierced in the parietal pleura through the tube lumen. The tubes were then connected to pressure transducers and the whole system was filled with heparinized saline to the level of the parietal pleura; finally the pneumo-thorax was removed after N2O washout and Pliq recordings were performed. A different kind of tube was used to obtain microsamples of pleural fluid (2.5-3 microliters) during spontaneous breathing; colloid osmotic pressure of the microsamples (pi liq) was measured with an osmometer, and averaged 9.3 +/- 1.5 cm H2o (n = 70 samples). When pooled and plotted against lung height end-expiratory intercostal and costal Pliq data scattered along a single regression line with a slope of -0.83 and -0.90 cm H2O cm(-1) in supine and prone animals, respectively. End-inspiratory costal Pliq was significantly more subatmospheric than intercostal in the ventral region of the chest (P < 0.05), and less subatmospheric in the dorsal region, regardless of posture. The techniques presented here could be helpful in gaining a greater insight into the physiology and pathophysiology of the pleural space in terms of pleural fluid dynamics and turnover.
Authors:
M Del Fabbro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental physiology     Volume:  83     ISSN:  0958-0670     ISO Abbreviation:  Exp. Physiol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-11-06     Completed Date:  1998-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  435-48     Citation Subset:  IM    
Affiliation:
Istituto di Fisiologia Umana I., Università degli Studi, Milano, Italy. massimo.delfabbro@unimi.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Extracellular Space / chemistry,  physiology*
Hydrostatic Pressure*
Kinetics
Lung / physiology*
Osmotic Pressure
Pleura / physiology*
Rabbits
Respiration
Thorax / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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